Model construction and clinical therapeutic potential of engineered cardiac organoids for cardiovascular diseases  

作　　者：Yongtao Wang Yan Hou Tian Hao Marta Garcia-Contreras Guoping Li Dragos Cretoiu Junjie Xiao 

机构地区：[1]Cardiac Regeneration and Ageing Lab,School of Medicine,Shanghai University,Shanghai,China [2]Institute of Cardiovascular Sciences,Shanghai Engineering Research Center of Organ Repair,Joint International Research Laboratory of Biomaterials and Biotechnology in Organ Repair(Ministry of Education),School of Life Science,Shanghai University,Shanghai,China [3]Cardiovascular Division of the Massachusetts General Hospital and Harvard Medical School,Boston,MA,USA [4]Department of Medical Genetics,Carol Davila University of Medicine and Pharmacy,Bucharest,Romania [5]Materno-Fetal Assistance Excellence Unit,Alessandrescu-Rusescu National Institute for Mother and Child Health,Bucharest,Romania

出　　处：《Biomaterials Translational》2024年第4期337-354,共18页生物材料转化电子杂志（英文）

基　　金：supported by National Natural Science Foundation of China(No.82302401 to YW);the grant from Science and Technology Commission of Shanghai Municipality(Nos.23410750100,20DZ2255400 and 21XD1421300 to JX);the“Dawn”Program of Shanghai Education Commission(No.19SG34 to JX).

摘　　要：Cardiovascular diseases cause significant morbidity and mortality worldwide.Engineered cardiac organoids are being developed and used to replicate cardiac tissues supporting cardiac morphogenesis and development.These organoids have applications in drug screening,cardiac disease models and regenerative medicine.Therefore,a thorough understanding of cardiac organoids and a comprehensive overview of their development are essential for cardiac tissue engineering.This review summarises different types of cardiac organoids used to explore cardiac function,including those based on co-culture,aggregation,scaffolds,and geometries.The self-assembly of monolayers,multilayers and aggravated cardiomyocytes forms biofunctional cell aggregates in cardiac organoids,elucidating the formation mechanism of scaffold-free cardiac organoids.In contrast,scaffolds such as decellularised extracellular matrices,three-dimensional hydrogels and bioprinting techniques provide a supportive framework for cardiac organoids,playing a crucial role in cardiac development.Different geometries are engineered to create cardiac organoids,facilitating the investigation of intrinsic communication between cardiac organoids and biomechanical pathways.Additionally,this review emphasises the relationship between cardiac organoids and the cardiac system,and evaluates their clinical applications.This review aims to provide valuable insights into the study of three-dimensional cardiac organoids and their clinical potential.

关 键 词：cardiac organoids cardiovascular diseases clinical applications scaffolds SELF-ASSEMBLY 

分 类 号：R318.1[医药卫生—生物医学工程]