基于16s rRNA测序研究长期高氧诱导的小鼠肠道菌群紊乱  

Long-term hyperoxia-induced intestinal dysbiosis in mice was studied based on 16s rRNA sequencing

作　　者：盛忠燕 骆燕洪 戴妮喃 肖建辉 邢周雄 Sheng Zhongyan;Luo Yanhong;Dai Ninan;Xiao Jianhui;Xing Zhouxiong(Department of Critical Care Medicine,People’s Hospital of Qiansouthwest Buyi and Miao Autonomous Prefecture,Xingyi Guizhou 562400,China;Department of Critical Care Medicine,Qianxinan Hospital Affiliated of Zunyi Medical University,Xingyi Guizhou 562400,China;First Clinical College,Zunyi Medical University,Zunyi Guizhou 563006,China;Department of Critical Care Medicine,Affiliated Hospital of Zunyi Medical University,Zunyi Guizhou 563000,China;Guizhou Provincial Key Laboratory of Medicinal Biotechnology in Colleges and Universities,Affiliated Hospital of Zunyi Medical University,Zunyi Guizhou 563000,China)

机构地区：[1]黔西南布依族苗族自治州人民医院重症医学科,贵州兴义562400 [2]遵义医科大学附属黔西南医院重症医学科,贵州兴义562400 [3]遵义医科大学第一临床学院,贵州遵义563006 [4]遵义医科大学附属医院重症医学科,贵州遵义563000 [5]遵义医科大学附属医院,贵州省高等学校医药生物技术重点实验室,贵州遵义563000

出　　处：《遵义医科大学学报》2024年第11期1069-1075,共7页Journal of Zunyi Medical University

基　　金：国家自然科学基金资助项目(NO:82160370);贵州省高等学校重点实验建设项目[NO:黔教技(2023020)];贵州省科技计划项目[NO:黔科合支撑(2022)一般179]。

摘　　要：目的通过氧气吸入建立小鼠长期高氧模型,用16s rRNA测序研究长期高氧暴露对小鼠肠道菌群结构的作用;并使用抗生素清扫(ABX)模型研究肠道菌群对高氧诱导肠损伤的调控作用。方法16只雄性C57BL/6小鼠随机分为高氧组和对照组,分别暴露于80%氧气和空气14 d,收集小鼠粪便进行16s rRNA测序。对ABX小鼠和普通SPF小鼠分别进行高氧造模,检测肠损伤指标。结果高氧使蓝细菌门和放线菌门显著增多,改变了菌群的β多样性。高氧组的丹毒丝菌科和放线菌门为标志菌群,而对照组的Muribaculaceae菌为标志菌群。ABX显著减轻了高氧诱导的肠损伤和免疫通路(LPS/TLR-4)激活。结论长期高氧诱导小鼠肠道菌群紊乱,使需氧菌增多并使专性厌氧菌减少。肠道菌群可能通过LPS/TLR-4信号通路调控高氧诱导的肠损伤。Objective A mouse model of long-term hyperoxia was established by oxygen inhalation,and the effects of long-term hyperoxia on intestinal microbiota were explored by 16s rRNA sequencing;antibiotic treated(ABX)model was also performed to investigate how gut microbiome modulate hyperoxia-induced gut injury.Methods 16 C57BL/6 male mice were randomly divided into hyperoxia and control groups exposed to 80%oxygen and room air for 14 days,respectively.Fecal pellets were collected for 16s RNA sequencing.In addition,ABX and SPF mice were exposed to hyperoxia and room air,respectively,and intestinal gut injury was analyzed.Results Hyperoxia significantly increased the cyanobacteria and actinobacteria,and altered theβdiversity of intestinal flora.Erysipelotrichaceae and actinobacteria in the hyperoxia group were the marker bacteria,while muribaculaceae in the control group were the marker bacteria.ABX significantly reduced the hyperoxia-induced gut injury and immune response(LPS/TLR-4).Conclusion Long-term hyperoxia induces gut dysbiosis in mice,increasing relative abundance of aerobes and decreasing obligate anaerobes.Gut microbiome may contribute to hyperoxia-induced gut injury through LPS/TLR-4 pathway.

关 键 词：静脉-动脉体外膜肺氧合 高氧 肠道菌群 厌氧菌 muribaculaceae TLR-4 

分 类 号：R379.1[医药卫生—病原生物学]