光合细菌沼泽红假单胞菌Atp2蛋白对水稻生长及抗逆的影响  

Effects of photosynthetic bacteria Rhodopseudomonas palustris Atp2 protein on growth and stress resistance in rice

作　　者：陈春燕 黄强 吴希阳 覃莹菲 谭新球[2] 刘勇[2] 陈岳 张德咏[1,2] CHEN Chun-yan;HUANG Qiang;WU Xi-yang;QIN Ying-fei;TAN Xin-qiu;LIU Yong;CHEN Yue;ZHANG De-yong(College of Plant Protection,Hunan Agricultural University,Changsha,Hunan 410125,China;Institute of Plant Protection,Hunan Academy of Agricultural Sciences,Changsha,Hunan 410125,China)

机构地区：[1]湖南农业大学植物保护学院,湖南长沙410125 [2]湖南省农业科学院植物保护研究所,湖南长沙410125

出　　处：《南方农业学报》2024年第9期2632-2640,共9页Journal of Southern Agriculture

基　　金：国家自然科学基金项目(31972323);湖南省科技创新计划项目(2021RC3114);湖南省农业科技创新资金项目(2023CX04)。

摘　　要：【目的】探究光合细菌沼泽红假单胞菌PSB06 Atp2蛋白对水稻生长、防御酶活性及防御相关基因转录水平的影响,为生防菌在水稻上的推广应用提供理论依据。【方法】以水稻品种CO-39为试验材料,用0(蒸馏水,CK)、20和50μg/mL Atp2蛋白3种处理液对水稻种子和叶片进行处理,对生长14d的水稻体内叶绿素a/b和过氧化氢(H_(2)O_(2))含量,以及过氧化物酶(POD)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、谷胱氨肽还原酶(GR)、苯丙氨酸解氨酶(PAL)、多酚氧化酶(PPO)等水稻防御酶活性进行检测;运用实时荧光定量PCR检测水稻体内防御相关基因(OsWRKY70、OsLOX1、OsAOS2、OsPR1a、OsCHT1和OsPAL1)的转录水平。【结果】与CK相比,Atp2蛋白处理水稻叶片后,其体内叶绿素a/b显著(P<0.05,下同)或极显著(P<0.01,下同)升高,而处理种子后叶绿素a/b无显著变化(P>0.05);Atp2蛋白处理后水稻体内H_(2)O_(2)含量均升高,其中处理叶片后H_(2)O_(2)含量极显著升高;Atp2蛋白处理后水稻体内POD和CAT活性显著或极显著下降;Atp2蛋白处理后水稻体内SOD、GR和PAL活性均极显著升高;Atp2蛋白处理种子后水稻体内PPO活性显著或极显著升高,而处理叶片后PPO活性显著或极显著下降。Atp2蛋白处理后水稻防御相关基因OsWRKY70、OsLOX1、OsAOS2(50μg/mL Atp2蛋白处理水稻叶片外)、OsPR1a和OsCHT1表达均上调,其中OsWRKY70、OsLOX1和OsCHT1基因表达显著或极显著上调;Atp2蛋白处理种子后OsPAL1基因表达极显著上调,而处理叶片后OsPAL1基因表达极显著下调。【结论】沼泽红假单胞菌PSB06 Atp2蛋白对水稻有较好的促生抗逆效果,具有推广应用潜力。【Objective】To investigate the effects of the photosynthetic bacteria Rhodopseudomonas palustris PSB06 Atp2 protein on the growth,defence enzyme activity and the transcript levels of defense-related genes in rice,and to provide theoretical basis for the popularization of the use of biocontrol bacteria in rice.【Method】Rice variety CO-39 was used as the test material,and rice seeds and leaves were treated with 3 treatment solutions of 0(distilled water,CK),20 and 50μg/mL Atp2 protein,the chlorophyll a/b and the hydrogen peroxide(H_(2)O_(2))content were detected,as well as the activities of rice defense-related enzymes such as peroxidase(POD),catalase(CAT),superoxide dismutase(SOD),glutathione reductase(GR),phenylalanine ammonia-lyase(PAL),polyphenol oxidase(PPO)in rice,and the transcript levels of defense-related genes(OsWRKY70,OsLOX1,OsAOS2,OsPR1a,OsCHT1 and OsPAL1)in rice were detected by real-time fluorescence quantitative PCR.【Result】Compared with CK,chlorophyll a/b was significantly(P<0.05,the same below)or extremely significantly(P<0.01,the same below)increased in rice after Atp2 protein treatment of rice leaves,while chlorophyll a/b did not change significantly after treatment of seeds(P>0.05);H_(2)O_(2) content in rice was ele-vated in all Atp2 protein treatments,with extremely significant elevation in the content of H_(2)O_(2) after treatment of leaves;POD and CAT activities in rice were significantly or extremely significantly decreased after Atp2 protein treatment;SOD,GR and PAL activities in rice were extremely significantly increased after Atp2 protein treatment;PPO activity in rice was significantly or extremely significantly increased after Atp2 protein treatment of seeds,whereas PPO activity was significantly or extremely significantly decreased after treatment of leaves.After Atp2 protein treatment,expressions of rice defense genes OsWRKY70,OsLOX1,OsAOS2(except 50μg/mL Atp2 protein treated rice leaves),OsPR1a and OsCHT1 were all up-regulated,of which the expression of OsWRKY70,OsLOX1 a

关 键 词：光合细菌 沼泽红假单胞菌 水稻 Atp2蛋白 防御酶活 防御相关基因 

分 类 号：S511[农业科学—作物学]