硝普钠诱导下川西獐牙菜的LC-MS非靶向代谢组分析  

LC-MS based non-targeted metabolomics analysis of Swertia mussotii Franch induced by sodium nitroprusside

作　　者：王文静 王易 张亚美[1] 杨璞 刘文英 何舒萍 向蓓蓓[1] WANG Wenjing;WANG Yi;ZHANG Yamei;YANG Pu;LIU Wenying;HE Shuping;XIANG Beibei(Tianjin University of Chinese Medicine,Tianjin 301617,China)

机构地区：[1]天津中医药大学,天津301617

出　　处：《湖南中医药大学学报》2024年第10期1857-1864,共8页Journal of Hunan University of Chinese Medicine

基　　金：国家自然科学基金项目(82074120)。

摘　　要：目的研究基于非靶向代谢组学技术的川西獐牙菜在硝普钠处理后的代谢响应,为相关研究提供参考。方法以1.0 mmol/L硝普钠处理的川西獐牙菜为实验组,蒸馏水处理的川西獐牙菜为对照组,对实验组和对照组的川西獐牙菜中差异代谢物进行主成分分析(principal component analysis,PCA)、正交偏最小二乘判别分析(orthogonal partial least squares discriminant analysis,OPLS-DA)以及KEGG富集分析。结果从实验组和对照组处理的川西獐牙菜中共检测到523种代谢物,通过OPLS-DA模型的数据分析,根据VIP>1和P<0.05条件共筛选出104种差异显著代谢物,其中有36种代谢物上调、68种代谢物下调,这些差异代谢物包括黄酮类、生物碱类、有机酸、氨基酸、糖类、维生素、香豆素类化合物及其他化合物。KEGG富集结果显示,对照组与实验组差异代谢物富集到121条途径中。结论推测川西獐牙菜的差异代谢物显著富集的12条代谢途径主要与抗氧化有关。Objective To study the metabolic response of Swertia mussotii Franch after sodium nitroprusside treatment based on non-targeted metabolomics technology,and to provide reference for related research.Methods The experimental group consisted of Swertia mussotii Franch treated with 1.0 mmol/L sodium nitroprusside,while the control group consisted of Swertia mussotii Franch treated with distilled water.Principal component analysis(PCA),orthogonal partial least squares discriminant analysis(OPLS-DA)and KEGG enrichment analysis were performed on the differential metabolites in the experimental group and the control group.Results A total of 523 metabolites were measured in Swertia mussotii Franch from the experimental group and the control group.Through the data analysis of the OPLS-DA model,104 significantly different metabolites were screened according to VIP>1 and P<0.05 conditions.Among them,36 metabolites were up-regulated and 68 metabolites were down-regulated.These differential metabolites include flavonoids,alkaloids,organic acids,amino acids,sugars,vitamins,coumarins compounds and other compounds.The results of KEGG enrichment analysis showed that the differential metabolites between the control group and the experimental group were enriched into 121 pathways.Conclusion It is speculated that 12 metabolic pathways of significantly enriched differential metabolites of Swertia mussotii Franch is mainly related to anti-oxidation.

关 键 词：川西獐牙菜 硝普钠 非靶向代谢组学 次生代谢 差异代谢物 抗氧化 

分 类 号：R285.5[医药卫生—中药学]