生物3D打印技术用于功能性骨骼肌3D组织构建的研究进展  

Research Progress of Biological 3D Printing Strategy in Three-Dimensional Tissue Construction of Functionalized Skeletal Muscle

作　　者：张飞虎 李婷 林智伟 李严兵 黄文华 Zhang Feihu;Li Ting;Lin Zhiwei;Li Yanbing;Huang Wenhua(Department of Basic Medical Sciences/Department of Human Anatomy and Tissue Embryology,Guangdong Medical University,Dongguan523808,Guangdong,China;State Key Discipline of Human Anatomy,School of Basic Medical Sciences,Southern Medical University,Guangzhou 510515,China;Guangdong Provincial Key Laboratory of Medical Biomechanics,Guangzhou 510515,China;Guangdong Medical 3D Printing Application Translational Engineering Technology Research Center,Guangzhou 510515,China)

机构地区：[1]广东医科大学基础医学院/人体解剖与组织胚胎学教研室,广东东莞523808 [2]南方医科大学基础医学院人体解剖学国家重点学科,广州510515 [3]广东省医学生物力学重点实验室,广州510515 [4]广东省医学3D打印应用转化工程技术研究中心,广州510515

出　　处：《中国生物医学工程学报》2024年第5期620-630,共11页Chinese Journal of Biomedical Engineering

基　　金：国家重点研发计划(2022YFB4600600);国家自然科学基金(32271181);南方医科大学高等教育教学改革项目(JG2020035)

摘　　要：骨骼肌作为机体运动系统的重要组成,对维持运动功能不可或缺。然而,体积性骨骼肌缺失(VML)所致运动功能丧失严重限制了机体的活动能力,并伴随多种并发症。当前,临床对VML的治疗主要依赖于移植手术,但其受限于供体部位发病率、供体组织缺乏以及对高技能手术团队的依赖。鉴于此,生物3D打印技术凭借其高效精准的特点,为功能性肌肉的制造以及VML的再生修复开辟了新的途径。综述了生物3D打印技术在功能化骨骼肌组织构建方面的研究现状,回顾了生物3D打印的多种技术方案,探讨了生物墨水在功能性肌肉构建中的应用进展。重点讨论了该技术在构建血管化和神经化骨骼肌仿生组织方面的最新方法,总结了各种3D打印构建的功能化骨骼肌结构对骨骼肌缺损修复的作用,最后分析了现有技术的局限性,展望了生物3D打印功能化骨骼肌结构领域的发展趋势.Skeletal muscle is essential for body movement,and loss of motor function due to volumetric muscle loss(VML)can limit the ability to move.Transplant surgery is the main clinical approach,however,is still limited by the morbidity of the donor site,the lack of donor tissue,and the need for a highly skilled surgical team.Biological 3D printing technology provides a more efficient and accurate method for the manufacture of functional muscles,and a feasible method for the regeneration and repair of VML muscles.This paper reviewed the research status of biological 3D printing technology in the construction of functional skeletal muscle tissue structure,as well as research progress of biological 3D printing and biological ink in functional muscle structures,mainly focusing on the construction of vascularized and neuralized skeletal muscle biomimetic tissue.The effects of various functional skeletal muscle structures constructed by 3D printing on the repair of skeletal muscle defects were summarized.Finally,development trends and existing limitations in the field were proposed.

关 键 词：生物3D打印 功能性骨骼肌 神经化/血管化肌肉 骨骼肌组织工程 体积性骨骼肌损失 

分 类 号：R318[医药卫生—生物医学工程]