Proanthocyanidins isolated from lotus seed skin mitigate glycolipid metabolism disorder through the p38/Nrf2/NF-κB signaling pathway  

作　　者：Yuhang Yi Tao Li Chenghao Lv Wenjiang He Wenzhi Li Xixin Zhou Si Qin 

机构地区：[1]College of Food Science and Technology,Hunan Agricultural University,Changsha 410128,China [2]Hunan Agricultural Products Processing Institute,Hunan Academy of Agricultural Sciences,Changsha 410125,China [3]College of Biological Science and Technology,Hunan Agricultural University,Changsha 410128,China [4]Infinitus(China)Company Ltd.,Guangzhou 510665,China

出　　处：《Acta Biochimica et Biophysica Sinica》2024年第9期1300-1310,共11页生物化学与生物物理学报（英文版）

基　　金：supported by the grants from the National Key Research and Development Program of China(No.2019YFC1604903);the Hunan Natural Science Foundation(No.2023JJ30295 to S.Q.).

摘　　要：Lotus seed skin extract is rich in flavonoids,making it a promising candidate for developing health products.In a previous study,we found that proanthocyanidins from lotus seed skin,particularly proanthocyanidin B1(PB1),can indirectly activate the Nrf2 signaling pathway,exerting an antioxidant effect.In this study,we isolate proanthocyanidins from lotus seed skin(PLS)using ethanol extraction and RP-HPLC identification,and investigate its effects on glycolipid metabolism both in vivo and in vitro.Our results demonstrate that PLS reduces body weight in high-fat diet(HFD)mice by decreasing feed efficiency.PLS also normalizes serum glucose,insulin secretion,glycosylated hemoglobin(HbA1c),and intraperitoneal glucose tolerance(IPGTT).Furthermore,PLS significantly improves blood lipid parameters and inhibits the expressions of six proinflammatory factors,including IL-1α,IL-1β,IL-3,IL-6,IFN-γand TNF-αin HFD mice.Additionally,analysis of fresh liver tissues reveals that PLS and PB1 induce the expressions of antioxidant proteins such as HO-1 and NQO1 by activating the p38-Nrf2 signaling pathway and inhibiting the NF-κB signaling pathway.In conclusion,proanthocyanidins from lotus seed skin regulate glycolipid metabolism disorders by targeting the p38/Nrf2/NF-κB signaling pathway.Our study offers a new approach for the high-value comprehensive utilization of lotus seed skin by-products and precise dietary intervention for metabolic syndrome.

关 键 词：PROANTHOCYANIDINS lotus seed skin p38/Nrf2/NF-kB glycolipid metabolism 

分 类 号：R285[医药卫生—中药学]