Role of Emerin in regulating fibroblast differentiation and migration at the substrate of stiffness coupled topology  

作　　者：Tiantian Yang Li Wang Haiyang Ma Kailun Li Yajing Wang Wenjie Tang Zichen Wang Meiwen An Xiang Gao Ludan Xu Yunyun Guo Jiqiang Guo Yong Liu Hugen Wang Yang Liu Quanyou Zhang 

机构地区：[1]College of Biomedical Engineering,Taiyuan University of Technology,Taiyuan 030024,China [2]Trauma Center,Trauma Orthopaedics,ZhouKou Orthopaedic Hospital,Zhoukou 466000,China [3]Shanxi Bethune Hospital,the Third Hospital of Shanxi Medical University,Taiyuan 030053,China [4]Dermatology Department,Shanxi Bethune Hospital,Shanxi Academy of Medical Sciences,Taiyuan 030032,China [5]Orthopaedics department,the First People’s Hospital of Jinzhong,Jinzhong 030600,China [6]Department of Nuclear Medicine,the First Hospital of Shanxi Medical University,Taiyuan 030012,China [7]Department of Orthopaedics,Shanxi Medical University,Taiyuan 030001,China

出　　处：《Acta Biochimica et Biophysica Sinica》2024年第9期1387-1400,共14页生物化学与生物物理学报（英文版）

基　　金：supported by the grants from the National Natural Science Foundation of China(Nos.12002232,12272251,12272252,31870934).

摘　　要：In hypertrophic scars,the differentiation and migration of fibroblasts are influenced by the extracellular matrix microenvironment,which includes factors such as stiffness,restraint,and tensile force.These mechanical stresses incite alterations in cell behavior,accompanied by cytoskeletal protein reorganization.However,the role of nucleo-skeletal proteins in this context remains underexplored.In this study,we use a polyacrylamide hydrogel(PAA)to simulate the mechanical stress experienced by cells in scar tissue and investigate the impact of Emerin on cell behavior.We utilize atomic force microscopy(AFM)and RNA interference technology to analyze cell differentiation,migration,and stiffness.Our findings reveal that rigid substrates and cellular restriction elevate Emerin expression and diminish differentiation.Conversely,reducing Emerin expression leads to attenuated cell differentiation,where stiffness and constraining factors exert no notable influence.Furthermore,a softening of cells and an enhanced migration rate are also markedly observed.These observations indicate that variations in nuclear skeletal proteins,prompted by diverse matrix microenvironments,play a pivotal role in the pathogenesis of hypertrophic scars(HSs).This research offers novel insights and a reference point for understanding scar fibrosis formation mechanisms and preventing fibrosis.

关 键 词：hyperplastic scar matrix microenvironment nuclear skeleton protein cell differentiation cell migration 

分 类 号：Q24[生物学—细胞生物学]