千金藤素微生物转化产物的初步研究  

Preliminary studies on the microbial transformation products of cepharanthine

作　　者：高元玮 杨郁 徐锐 赵雪 宫鹤翔 康亚帅 单俊杰 李长伟 GAO Yuanwei;YANG Yu;XU Rui;ZHAO Xue;GONG Hexiang;KANG Yashuai;HAN Junjie;LI Changwei(School of Pharmacy,Nanjing University of Chinese Medicine,Nanjing 210046,China;Institute of Pharmacology and Toxicology,Academy of Military Medical Sciences,Beijing 100850,China;School of Chinese Materia Medica,Shenyang Pharmaceutical University,Shenyang 110016,China)

机构地区：[1]南京中医药大学药学院,江苏南京210046 [2]军事医学研究院毒物药物研究所,北京100850 [3]沈阳药科大学中药学院,辽宁沈阳110016

出　　处：《中国药物化学杂志》2024年第5期374-381,共8页Chinese Journal of Medicinal Chemistry

摘　　要：目的筛选能够对千金藤素进行生物转化的真菌菌株,并对转化得到的千金藤素衍生物进行分离鉴定。方法采用液体发酵法将待选菌株与千金藤素共培养得到发酵产物,采用TLC和HPLC等分析方法对发酵产物进行分析,根据分析结果筛选得到目标菌株。采用多种色谱分离技术分离纯化千金藤素转化产物,根据理化性质和波谱(质谱、核磁共振谱)数据鉴定化合物结构。结果与结论筛选得到能够对千金藤素进行生物转化的目标菌株YNJ9和YNJ135,选取菌株YNJ9(Penicillium camponotum)对千金藤素进行发酵,从转化产物中分离出3个化合物,分别鉴定为2-norcepharanthine hydrochloride、cepharanthine-2-α-N-oxide和cepharanthine-2′-α-N-oxide。本文作者首次报道了千金藤素的微生物转化研究,为千金藤素及其衍生物的进一步研究提供了新思路。In this study,a liquid fermentation technique was employed to identify fungal strains capable of biotransforming cepharanthine,resulting in the isolation of multiple derivatives.The fermentation products were obtained through co-culturing the selected strains with cepharanthine utilizing this method.The products were analyzed using TLC and HPLC to screen the target strains.Two strains,YNJ9 and YNJ135,with cepharanthine transformation capabilities were screened,and strain Penicillium camponotum YNJ9 was chosen for further investigation of cepharanthine transformation products.The conversion products were isolated using multiple separation techniques and identified based on their physicochemical properties and spectral data as 2-norcepharanthine hydrochloride(1),cepharanthine-2-α-N-oxide(2)and cepharanthine-2′-α-N-oxide(3).This study marks the first report on the microbial transformation of cepharanthine,offering novel insights for further exploration of cepharanthine and its derivatives.

关 键 词：微生物转化 千金藤素 Penicillium camponotum 

分 类 号：R284[医药卫生—中药学]