黄芪多糖介导NF-κB/SREBP-2信号通路抑制肝脏胆固醇代谢改善小鼠冠心病  

Astragalus polysaccharide on inhibiting liver cholesterol metabolism by mediating NF-κB/SREBP-2 signaling pathway and improving coronary heart disease in mice

作　　者：杜丞禹 陈松 吕伦杰 刘永兴[2] DU Cheng-yu;CHEN Song;LV Lun-jie;LIU Yong-xing(Department 2 of Internal Medicine,Cangxian Hospital,Cangzhou 061009;Department 2 of Cardiology,Cangzhou Central Hospital,Cangzhou 061017,China)

机构地区：[1]沧县医院内二科,河北沧州061009 [2]沧州市中心医院心内二科,河北沧州061017

出　　处：《解剖科学进展》2024年第4期367-370,374,共5页Progress of Anatomical Sciences

基　　金：沧州市重点研发计划自筹项目。

摘　　要：目的探讨黄芪多糖(AP)能否通过抑制肝脏胆固醇代谢改善小鼠冠心病及对NF-κB-SREBP-2通路的影响。方法40只SPF级C57BL/6小鼠随机分为对照组(Control组)、模型组(CHD组)以及AP低、中、高剂量组,每组8只。除Control组外其余小鼠均通过喂食高脂饲料以及注射垂体后叶素构建CHD模型。油红O染色观察各组小鼠主动脉和肝脏组织形态,生化分析仪检测各组小鼠血脂四项以及胆固醇含量,qPCR检测各组小鼠肝脏组织中胆固醇代谢相关基因水平;Western blot法测定各组小鼠肝脏组织中p-NF-κB以及SREBP-2蛋白含量。结果与CHD组比较,不同剂量AP均能减少CHD小鼠主动脉斑块面积,降低血脂水平,降低肝脏中胆固醇含量,抑制胆固醇代谢相关基因水平,以及肝脏组织p-NK-κB和SREBP-2蛋白含量,且呈剂量依赖性。结论AP可通过抑制肝脏胆固醇代谢改善小鼠冠心病,这可能与抑制NF-κB-SREBP-2通路有关。Objective To investigate whether astragalus polysaccharide(AP)can improve coronary heart disease in mice by inhibiting liver cholesterol metabolism and its effect on NF-κB-SREBP-2 pathway.Methods Forty SPF grade C57BL/6 mice were randomly divided into Control group(Control group),model group(CHD group)and low,medium and high dose AP groups,with 8 mice in each group.Except the control group,the other mice were fed high-fat diet and injected pituitrin to construct CHD model.The tissue morphology of aorta and liver of mice in each group was observed by oil red O staining,the contents of four items of blood lipids and cholesterol of mice in each group were detected by biochemical analyzer,and the levels of genes related to cholesterol metabolism in liver tissues of mice were detected by qPCR.The contents of p-NF-κB and SREBP-2 protein in liver tissues of mice in each group were determined by Western blot.Results Compared with CHD group,different doses of AP group reduced the area of aortic plaque,reduced the level of blood lipid,reduced the content of cholesterol in liver,inhibited the level of cholesterol metabolism related genes,and the expressions of p-NF-κB and SREBP-2 in CHD mice.Conclusion Astragalus polysaccharides can improve CHD in mice by inhibiting liver cholesterol metabolism,which may be related to the inhibition of NF-κB-SREBP-2 pathway.

关 键 词：黄芪多糖 肝脏胆固醇代谢 冠心病 NF-κB-SREBP-2通路 

分 类 号：R541.4[医药卫生—心血管疾病]