S100A8在牙周炎症进程中的表达  

Expression of S100A8 in periodontal inflammatory process

作　　者：盛振娴 王雨欣 孙淑莉 于西佼[2,3] SHENG Zhenxian;WANG Yuxin;SUN Shuli;YU Xijiao(Binzhou Medical University,Yantai 264003,Shandong,P.R.China;Jinan Central Laboratory of Stomatological Hospital,Jinan 250001,Shandong,P.R.China;Key Laboratory of Shandong Oral Diseases and Tissue Regeneration,Jinan 250001,Shandong,P.R.China;Linzi Stomatological Hospital,Zibo 255499,Shandong,P.R.China)

机构地区：[1]滨州医学院,山东烟台264003 [2]济南市口腔医院中心实验室,山东济南250001 [3]山东省医药卫生口腔疾病与组织再生重点实验室,山东济南250001 [4]临淄区口腔医院,山东淄博255499

出　　处：《滨州医学院学报》2024年第5期360-363,共4页Journal of Binzhou Medical University

基　　金：山东省自然科学基金面上项目(ZR2020MH182);山东省医药卫生科技发展项目(202208010182);济南市口腔医院院长助研基金(202102)。

摘　　要：目的 探究在牙周炎牙周组织中S100A8的表达,为其在牙周炎中作用机制的研究提供一定的理论基础。方法 选取6例进行牙周手术的牙周炎患者,另选取6例牙龈健康的成人,将进行牙周手术的患者及健康牙龈切除术的患者分为牙周炎组(n=6)和正常组(n=6)两组,对其切除的牙龈进行免疫组织化学染色,观察其中S100A8的表达情况。体外培养人牙周膜细胞,1μg/mL脂多糖(lipopolysaccharide, LPS)刺激诱导炎性微环境,细胞免疫荧光染色以及实时定量PCR检测人牙周膜细胞中S100A8的基因及蛋白表达水平。体外培养小鼠单核巨噬细胞白血病细胞(小鼠RAW264.7细胞),1μg/mL LPS刺激诱导炎性微环境,实时定量PCR检测观察小鼠RAW264.7细胞中S100A8生物基因表达水平。结果 正常组牙龈上皮细胞可见S100A8表达,牙周炎组牙龈上皮及固有层结缔组织中均可见炎症细胞浸润,其中可见S100A8表达。牙周炎组S100A8阳性表达的炎症细胞数量明显多于正常组(P<0.05)。S100A8在人牙周膜细胞中阳性表达,1μg/mL LPS刺激培养24 h后,S100A8表达降低。人牙周膜细胞在LPS刺激24、72 h后,S100A8基因表达水平均较对照组有所降低,且表达水平与LPS刺激持续时间有相关性;小鼠RAW264.7细胞在1μg/mL LPS刺激培养24、72 h后,S100A8基因表达水平较对照组有所升高。结论 S100A8参与牙周炎的发生发展过程。在炎症环境下,其在炎症细胞中的表达升高,在牙周膜细胞中的表达降低。S100A8可能参与牙周膜细胞在牙周炎症过程中的调控,其作用机制有待于进一步探索。Objective To explore the expression of S100A8 in periodontitis tissues and to provide a theoretical basis for the study its mechanism in periodontitis.Methods We recruited 6 patients with periodontitis who were undergoing periodontal surgery,except those with severe systemic disease,and 6 adults with healthy gingival tissue.Patients and adults with healthy gingival tissue were divided into two groups:the periodontitis group(n=6)and the control group(n=6).Immunohistochemical staining was performed on the gingival tissues to observe the expression of S100A8.Human periodontal ligament cells were cultured in vitro and stimulated by 1μg/mL LPS to induce inflammatory environment.The gene expression level of S100A8 in these cells was observed through immunofluorescence staining and real-time PCR.Mouse mononuclear macrophage leukemia cells(mouse 264.7 RAW)were cultured in vitro and stimulated by 1μg/mL(lipopolysaccharide,LPS)to induce inflammatory environment to observe its gene expression level of S100A8 by real-time PCR.Results Immunohistochemical staining results showed S100A8-positive expression of the gingival epithelial cells of the control group,and inflammatory cell infiltration and S100A8-positive expression in gingival epithelium and connective tissue of the lamina in the periodontitis group.Immunofluorescence staining showed S100A8-positive expressed in human periodontal ligament cells and the expression level of S100A8 decreased after 1μg/mL LPS stimulation for 24 hours.Quantitative PCR results showed that the expression level of S100A8 gene in human periodontal ligament cells after LPS stimulation for 24 hours and 72 hours was lower than that in the control group,and the expression level was correlated with the duration of LPS stimulation.After cultured with 1μg/mL LPS for 24 hours and 72 hours,the expression level of S100A8 gene in RAW264.7 cells was higher than that in the control group.Conclusion S100A8 is involved in the occurrence and development of periodontitis.In the inflammatory environment,its

关 键 词：牙周炎 S100A8 牙周内环境 脂多糖 

分 类 号：R780.2[医药卫生—口腔医学]