利用QTL-Seq结合分子标记定位粳稻垩白粒率控制位点qChalk8  

Mapping of qChalk8 Controlling Chalky Rice Rate in japonica Rice by Combining QTL-Seq with Molecular Markers

作　　者：杜彦修[1] 孙文玉 袁泽科 张倩倩 李富豪 李俊周[1] 孙红正[1] DU Yanxiu;SUN Wenyu;YUAN Zeke;ZHANG Qianqian;LI Fuhao;LI Junzhou;SUN Hongzheng(College of Agronomy,Henan Agricultural University,Zhengzhou 450046)

机构地区：[1]河南农业大学农学院,郑州450046

出　　处：《中国水稻科学》2024年第6期665-671,共7页Chinese Journal of Rice Science

基　　金：河南省自然科学基金资助项目(232300421156);河南现代农业水稻产业技术体系项目(HARS-22-03-G3)。

摘　　要：[目的]垩白是影响稻米外观品质的重要性状。本研究旨在利用QTL-Seq和分子标记作图定位粳稻垩白粒率调控相关的QTL。[方法]利用高垩白粒率粳稻材料拉木加和低垩白粒率粳稻品种水晶3号构建F_(2)分离群体,将两份独立的F_(2)群体分别种植于河南原阳和海南三亚,两个群体单株垩白粒率考种后分别选取极端个体混池进行QTL-Seq分析,然后使用分子标记作图对稳定的QTL进行验证。[结果]两个F_(2)群体的QTL-Seq分析发现8号染色体存在一个较为稳定的QTL位点qChalk8。进一步通过分子标记作图将该QTL定位于17.6-18.5Mb,该QTL位点的LOD值为7.08,表型贡献率为15.9%。[结论]利用QTL-Seq和分子标记作图定位了粳稻垩白粒率相关QTL-qChalk8,为粳稻垩白调控基因进一步的精细定位和基因克隆奠定了基础。[Objective]Chalkiness is an important characteristic that affects the appearance quality of rice grains.This study aims to use QTL-Seq and molecular marker mapping methods to locate QTLs related to chalky rice rates in japonica rice.[Method]F_(2) segregating populations were constructed using the japonica germplasm Lamujia with a high chalky rice rate and the japonica variety Shuijing 3 with a low chalky rice rate.Two independent F_(2) populations were planted in Yuanyang,Henan Province,and Sanya,Hainan Province,respectively.The single-plant chalky rice rate was examined,and extreme individuals were pooled for QTL-Seq analysis.Reliable QTLs were then subjected to verification using molecular marker mapping.[Result]QTL-Seq analysis of the two F_(2) populations revealed the existence of a stable chalky rice rate QTL on chromosome 8,designated as qChalk8.Further molecular marker mapping confined the QTL to a genomic region between 17.6 Mb and 18.5 Mb.The LOD score for qChalk8 was 7.08,and qChalk8 could explain 15.9% of the phenotypic variation.[Conclusion]QTL-Seq and molecular marker mapping successfully identified qChalk8 related to chalky rice rates in japonica rice.The localization of qChalk8 establishes a foundation for further fine mapping and gene cloning of the chalkiness regulatory gene in japonica rice.

关 键 词：水稻 垩白 垩白粒率 QTL-Seq QTL定位 

分 类 号：S511[农业科学—作物学]