枯草芽孢杆菌对兰州百合生长发育及鳞茎代谢的影响  

Effects of Bacillus subtilis on the Growth and Bulb Metabolism of Lilium davidii var.unicolor

作　　者：陈雨欣 马祥臣 王健铭 张萍[1] 靳磊 CHEN Yuxin;MA Xiangchen;WANG Jianming;ZHANG Ping;JIN Lei(College of Forestry and Prataculture,Ningxia University,Yinchuan 750021)

机构地区：[1]宁夏大学林业与草业学院,银川750021

出　　处：《植物研究》2024年第6期926-935,共10页Bulletin of Botanical Research

基　　金：宁夏回族自治区大学生创新创业训练计划项目(S202310749107);国家自然科学基金项目(32260727);宁夏自治区重点研发项目(2021BBF02025)。

摘　　要：为探究枯草芽孢杆菌(Bacillus subtilis)对兰州百合(Lilium davidii var. unicolor)生长发育及鳞茎代谢物的影响,以兰州百合为试验材料,设置CK(泥炭+珍珠岩)、TR(泥炭+珍珠岩+枯草芽孢杆菌)2组处理,采用灌根的方式施用枯草芽孢杆菌,观测不同时期(现蕾期、开花期、半枯期)兰州百合生长变化,并采用LC-MS法检测分析半枯期兰州百合鳞茎中差异代谢物。结果表明:与CK处理相比,TR处理对兰州百合的株高、茎粗、叶片数量、叶绿素含量、基生根数量、茎生根数量和鳞茎周径都有一定的促进作用。相较于CK处理,TR处理的株高和茎粗在现蕾期表现出显著差异(P<0.05),分别增加了20.06%和40.68%;叶片数量在现蕾期和开花期显著提高(P<0.05),达到CK处理的1.35倍和1.45倍;在开花期,TR处理的兰州百合鳞茎周径和茎生根数量相较于CK处理分别增加了15.87%和58.14%,差异显著(P<0.05)。在鳞茎代谢物分析中,通过CK组与TR组对比从兰州百合鳞茎中筛选出408种差异代谢物,富集的代谢途径有14条,其中差异代谢物主要富集在氨基酸代谢和合成通路。综上,配施枯草芽孢杆菌能够促进兰州百合生长发育,并影响鳞茎中营养物质代谢。In order to investigate the effect of Bacillus subtilis on the growth and development and its bulb metabolites of Lilium davidii var.unicolor,L.davidii var.unicolor was used as the experimental material,CK(peat+perlite)and TR(peat+perlite+B.subtilis)treatments were conducted using B.subtilis by root irrigation.The growth changes of L.davidii var.unicolor at different stages(budding period,flowering period and semi-dry period)were observed,the differential metabolites in bulbs in semi-dry period were detected and analyzed by LC-MS method.The results showed that compared with CK,TR treatment had promoting effects on plant height,stem diameter,number of blades,chlorophyll content,basal root number,stem root number and circumference of L.davidii var.unicolor,respectively.Compared with CK,the plant height and stem diameter under TR treatment showed significant differences(P<0.05)at the budding period,which increased by 20.06%and 40.68%,respectively.The number of leaves increased significantly at budding period and flowering period(P<0.05),reaching 1.35 times and 1.45 times of CK,respectively.At the flowering period,compared with CK,the circumference of bulbs and the number of rooting of L.davidii var.unicolor.stems treated with TR were increased by 15.87%and 58.14%,respectively,and the difference was significant(P<0.05).In the bulb metabolites analysis,408 differential metabolites were screened from the bulbs in CK vs TR groups.A total of 14 metabolic pathways were enriched,and the differential metabolites were mainly enriched in amino acid metabolism and synthesis pathways.In summary,the application of B.subtilis could promote the growth and development of L.davidii var.unicolor and affect the metabolism of nutrients in the bulbs.

关 键 词：兰州百合 枯草芽孢杆菌 农艺性状 差异代谢物 

分 类 号：Q949.71[生物学—植物学]