降低常夏石竹玻璃化率及促进生根的方法  

Methods for Reducing the Vitrification Rate and Promoting Rooting Formation of Dianthus plumarius

作　　者：田旭平[1] 王佳丽 乔珂 刘慧欣 岳康杰 TIAN Xuping;WANG Jiali;QIAO Ke;LIU Huixin;YUE Kangjie(College of Forestry,Shanxi Agricultural University,Taigu 030801)

机构地区：[1]山西农业大学林学院,太谷030801

出　　处：《植物研究》2024年第6期954-962,共9页Bulletin of Botanical Research

基　　金：山西农业大学科技创新基金项目(2020BQ37);山西农业大学研究生实践创新项目(2024SJ149)。

摘　　要：以常夏石竹(Dianthus plumarius)带芽茎段为外植体,筛选最佳HgCl_(2)消毒时间和增殖培养基,研究外加活性炭(AC)、矮壮素(CCC)、聚乙烯吡咯烷酮(PVP)、多效唑(PP_(333))对常夏石竹无菌苗玻璃化及不同植物生长激素配比对生根的影响。结果表明:适宜常夏石竹带芽茎段消毒的方法是75%乙醇消毒30 s+0.1%HgCl_(2)消毒10 min,成活率达86.63%;MS+2 mg·L^(-1)6-BA+0.05 mg·L^(-1)NAA+30 g·L^(-1)蔗糖+7 g·L^(-1)琼脂为适宜的增殖培养基,丛生芽增殖率为90.28%,平均高度为5.25 cm,增殖系数较高(12.31);在降低常夏石竹无菌苗玻璃化效果方面,添加物效力由高到低依次为CCC、AC、PP_(333)、PVP,在1/2MS培养基内添加3 mg·L^(-1)的CCC有利于无菌苗克服玻璃化,且植株生长健壮;在克服玻璃化率最优的培养基配方中添加0.5 mg·L^(-1)NAA适宜常夏石竹无菌苗生根,生根率达100%,平均根长为16.83 cm,单株平均生根数为15.66条。研究结果将为常夏石竹组培快繁及工厂化育苗提供理论和技术支持。The shoot tips of Dianthus plumarius were used as explants,and the optimal disinfection duration with HgCl·and the suitable proliferation medium were screened out.The effects of adding activated carbon(AC),chlormequat(CCC),polyvinylpyrrolidone(PVP),and paclobutrazol(PP_(333))on the vitrification aseptic seedlingsof D.plumarius,as well as the impact of different plant hormone ratios on rooting were investigated.The results indicated that the appropriate disinfection method for shoot tips of D.plumarius was 75%ethanol for 30 s followed by 0.1%HgCl_(2) for 10 min,and the survival rate was 86.63%.The suitable proliferation medium was identified as MS supplemented with 2 mg·L^(-1)6-benzylaminopurine(6-BA),0.05 mg·L^(-1) Naphthaleneacetic acid(NAA),30 g·L^(-1) sucrose,and 7 g·L^(-1) agar,resulting in cluster buds proliferation rate of 90.28%,with an average bud height of 5.25 cm and a high proliferation coefficient of 12.31.In reducing the vitrification effect aseptic seedlingsof D.plumarius,the effect from high to low was CCC,AC,PP_(333) and PVP.Adding 3 mg·L^(-1) CCC in 1/2 MS medium was beneficial for overcoming the vitrification phenomenon and promoting plant growth.Adding 0.5 mg·L·1 NAA to the medium formula that is optimal for overcoming the vitrification rate is appropriate for the rooting of sterile seedlings of D.plumarius semperflorens.The rooting rate can reach 100%,with an average root length of 16.83 cm and an average number of 15.66 roots per individual plant.The findings provided theoretical and technical supports for the rapid propagation and industrial seedling production of D.plumarius.

关 键 词：常夏石竹 消毒 增殖 玻璃化 生根 

分 类 号：Q949.745.8[生物学—植物学]