元宝槭幼苗光合生理的水氮耦合效应研究  

Study on the coupling effect of water and nitrogen on photosynthetic physiology of Acer truncatum seedlings

作　　者：王雨晴 裴顺祥[2] 李晓刚 辛学兵[2] 吴迪[2] 王玥 WANG Yuqing;PEI Shunxiang;LI Xiaogang;XIN Xuebing;WU Di;WANG Yue(College of Forestry,Heibei Agricultural University,Baoding 071000,China;Beijing Jiulong Mountain National Long-term Scientific Research Base of Warm Temperate Forests,Experimental Center of Forestry in North China,Chinese Academy of Forestry,Beijing 102300,China)

机构地区：[1]河北农业大学林学院,河北保定071000 [2]中国林业科学研究院华北林业实验中心,北京九龙山暖温带森林国家长期科研基地,北京102300

出　　处：《林业与生态科学》2024年第4期371-379,共9页Forestry and Ecological Sciences

基　　金：中国林业科学研究院中央级公益性研所本业务费专资重点基金项目(CAFYBB2020ZB005);科技部基础资源调查专项(2019FY100802_02)。

摘　　要：研究元宝槭幼苗光合生理的水氮耦合效应可以为元宝槭苗木水肥管理提供科学依据,有利于元宝槭产业发展。以1 a生元宝槭实生苗为材料,设置田间持水量(FC)的10%~20%(W1)、40%~50%(W2)、70%~80%(W3)3个水分梯度,施氮0 g/株(N0)、2 g/株(N1)、4 g/株(N2)和6 g/株(N3)4个氮肥梯度的二因素完全随机盆栽实验,通过测定不同水氮处理下元宝槭幼苗的光合参数、叶绿素含量、叶绿素荧光参数,分析其变化规律,揭示水氮耦合对元宝槭苗期光合生理的影响机制。结果表明,水氮耦合对元宝槭的光合生理有显著影响:(1)元宝槭幼苗的Pn、Gs、Ci、Tr等4个光合参数总体变化趋势表现为随着土壤含水量的增加而增加,而iWUE、LS则与之相反;施氮对元宝槭光合参数的影响较复杂,W2N2处理Pn最高,为8.84μmol/(m^(2)·s)。(2)水氮对元宝槭幼苗的光系统II的实际光化学效率(ΦPSII)、非光化学猝灭系数(qN)影响较大,而对光系统II的最大光化学效率(Fv/Fm)、光化学猝灭系数(qP)无明显影响。(3)水分是元宝槭叶绿素含量的主要影响因素,随土壤水分含量升高,元宝槭总叶绿素含量提高,叶绿素a/b值下降,叶绿素b的比例显著增大。(4)元宝槭Pn与Ci、Gs、Tr、Fv/Fm、Chl显著正相关,与iWUE、LS则呈现出显著负相关性。综上所述,提高土壤含水量能显著提高元宝槭幼苗的光合作用,土壤含水量较低时,水分是影响元宝槭光合生理的主导因素,土壤含水量为中、高水平时,其光合生理由水氮共同决定,该结论可为元宝槭苗期水肥管理体系建立提供理论参考。The research of the coupling effect of water and nitrogen on the photosynthetic physiology of Acer truncatum seedlings can provide a scientific basislay a scientific foundation for the water and fertilizer management of A.truncatum seedlings,which is beneficial to the development of A.truncatum industry.In this study,one-year-old A.truncatum seedlings were used as materials,and a two-factor completely randomized pot experiment was conducted with three water gradients of 10%~20%(W1),40%~50%(W2),and 70%~80%(W3)of field capacity(FC),and four nitrogen gradients of 0 g/plant(N0),2 g/plant(N1),4 g/plant(N2),and 6 g/plant(N3)of nitrogen fertilizer.The photosynthetic parameters,chlorophyll content,and chlorophyll fluorescence parameters of A.truncatum seedlings under different water and nitrogen treatments were measured to analyze their variation and reveal the influence mechanism of water and nitrogen coupling on the photosynthetic physiology of A.truncatum seedlings.The results showed that water and nitrogen coupling had a significant impact on the photosynthetic physiology of A.truncatum:(1)The overall trend of Pn,Gs,Ci,and Tr of A.truncatum seedlings showed an increase increased with the increasinge of soil water content,while iWUE and LS showed the opposite trend;the effect of nitrogen application on the photosynthetic parameters of A.truncatum was more complex,with the highest Pn of 8.84μmol/(m^(2)·s)in the W2N2 treatment.(2)Water and nitrogen had a significant impact on the actual photochemical efficiency of photosystem II(ΦPSII),and non-photochemical quenching coefficient(qN)of A.truncatum seedlings,while there was no significant effect on the maximum photochemical efficiency of photosystem II(Fv/Fm)and photochemical quenching coefficient(qP).(3)Soil moisture is the main influencing factor of chlorophyll content in A.truncatum leaves.With the increase of soil moisture content,the total chlorophyll content of A.truncatum leaves increasesd,the chlorophyll a/b ratio decreasesd,and the proportion of chlorophyll b

关 键 词：元宝槭 水氮耦合 光合作用 叶绿素荧光 

分 类 号：Q945.5[生物学—植物学]