异源三倍体(大果榆×四倍体白榆)榆树鉴定及代谢组学分析  

Identification and metabolomic analysis of allotriploid elm(Ulmus macrocarpa×tetraploid Ulmus pumila)

作　　者：闫晓丽 王书柔 丁新宇 刘兴菊[1] 梁海永[1] YAN Xiaoli;WANG Shurou;DING Xinyu;LIU Xingju;LIANG Haiyong(College of Forestry,Hebei Agricultural University,Baoding 071000,China)

机构地区：[1]河北农业大学林学院,河北保定071000

出　　处：《林业与生态科学》2024年第4期432-439,共8页Forestry and Ecological Sciences

基　　金：河北农业大学科研发展基金计划项目(1513007)。

摘　　要：为了探究大果榆与四倍体白榆杂交子代倍性及其与亲本之间差异代谢产物的遗传规律,以大果榆与四倍体白榆杂交获得的异源三倍体榆树子代及其亲本为材料,采用流式细胞仪进行倍性鉴定,并通过非靶代谢组学对亲本与榆树异源三倍体子代代谢物差异进行分析。结果表明:(1)杂交获得的子代为异源三倍体。(2)榆树异源三倍体与亲本之间差异代谢产物在数量及含量上存在显著差异,共筛选出差异代谢物121个,其中顺式-乌头酸、D-葡萄糖醛酸以及磷酸丝氨酸等代谢物相对含量高于亲本;皮甾酮、(R)-泛酰酸内酯以及甘露二糖等代谢物相对含量低于亲本;代谢物相对含量介于亲本之间的为甲基丁香酚、杂色曲菌素以及托卡尼等。(3)异源三倍体榆树与亲本差异代谢机制主要富集在脂肪酸代谢途径以及苯丙烷类生物合成代谢途径。异源三倍体榆树的主要代谢物与亲本间存在显著差异,代谢物的数量及含量与其表型、生理以及非生物胁迫抗性密切相关。In order to investigate the inheritance pattern of ploidy in the offspring of crosses between Ulmus macrocarpa and tetraploid Ulmus pumila and their differential metabolites from the parents,heterozygous triploid U.pumila offspring obtained from crosses between diploid U.macrocarpa and tetraploid U.Pumila and their parents were used for ploidy identification by flow cytometry,and metabolite differences between parental and heterozygous triploid U.pumila offspring were analyzed by non-target metabolomics.The results showed that:(1)The filial generation obtained by hybridization were allotriploid.(2)There were significant differences in the number and content of the different metabolites between the elm heterozygous triploids and the parents.A total of 121 metabolites were screened,including cis-aconitic acid,D-glucuronic acid and phosphoserine the content which were higher than those of the parents;Cortexolone,(R)-pantolactone and mannobiose were lower than those of the parents;And the contents methyl eugenol,versicolorone and tocainide were between those of the parents,which were intermediate to those of the parents.(3)The metabolic mechanisms that differentiate heterozygous triploid U.pumila from its parents mainly lied in the fatty acid metabolic pathway and the phenol propane biosynthetic metabolic pathway.The major metabolites of heterozygous triploid U.pumila differed significantly from those of the parents,and the quantity and content of metabolites were closely related to their phenotypic,physiological and abiotic stress resistance.

关 键 词：榆树 异源三倍体 代谢组学 代谢产物 

分 类 号：S722.3[农业科学—林木遗传育种]