杨梅素通过调控NF-κB和炎症因子对肝脏缺血再灌注损伤的作用研究  

Effects of Myricetin on Hepatic Ischemia-reperfusion Injury by Regulating NF-κB and Inflammatory Factors

作　　者：黄兴琼 高满军 张丹丹 陈迷 李炎坤[1,2] HUANG Xing-qiong;CHEN Mi;LI Yan-kun(School of Pharmacy,Xianning Medical College,Hubei University of Science and Technology,Xianning Hubei 437100,China)

机构地区：[1]湖北科技学院医学部药学院,湖北咸宁437100 [2]湖北科技学院糖尿病心脑血管病变湖北省重点实验室

出　　处：《湖北科技学院学报（医学版）》2024年第6期467-471,共5页Journal of Hubei University of Science and Technology(Medical Sciences)

基　　金：湖北自然科学基金(2024AFB494);湖北科技学院博士科研启动基金(BK202336);湖北科技学院横向科研经费(2023HX224)。

摘　　要：目的观察杨梅素(Myr)对肝脏缺血再灌注损伤(HIRI)炎性因子的作用。方法将C57BL/6小鼠分为4组,手术组(Sham)、缺血再灌注组(HIRI)、低剂量杨梅素组(HIRI-Myr-L)和高剂量杨梅素组(HIRI-Myr-H),建立肝脏缺血再灌注损伤模型。测定谷丙转氨酶(ALT)和谷草转氨酶(AST);HE染色检测各组小鼠肝组织形态情况;Western blot和qRT-PCR检测各组肝组织中核转录因子-κB(NF-κB)、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)的蛋白及mRNA表达情况。结果与HIRI组比较,HIRI-Myr-L、HIRI-Myr-H组ALT、AST水平均明显下降(P均<0.05);肝脏缺血面积明显减少,肝细胞肿胀变形减少;NF-κB、IL-6、TNF-α、IL-1β蛋白及mRNA表达水平降低(P均<0.05)。结论杨梅素能够抑制小鼠肝脏缺血再灌注诱导的NF-κB以及IL-6、TNF-α、IL-1β等炎症因子的表达升高,缓解肝脏损伤。Objective To observe the effect of myricetin(Myr)on inflammatory factors in hepatic ischemia-reperfusion injury(HIRI).Methods C57BL/6 mice were divided into four groups:surgery group(Sham),ischemia-reperfusion group(HIRI),low-dose myricetin group(HIRI-Myr-L),and high-dose myricetin group(HIRI-Myr-H).A hepatic ischemia-reperfusion injury was established.Alanine aminotransferase(ALT)and aminotransferase(AST)were measured;HE staining was used to detect the morphology of liver tissue in each group of mice;Western Blot and qRT-PCR were used to detect the protein and mRNA expression of nuclear transcription factor-κB(NF-κB),interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),and interleukin-1β(IL-1β)in liver tissues of each group.Results Compared with the results of the HIRI group,in the HIRI-Myr-L and HIRI-Myr-H groups the ALT and AST levels were significantly decreased(P<0.05),the ischemic area of the liver was significantly,the swelling and deformation of hepatocytes were reduced,and the protein and mRNA expression levels of NF-κB,IL-6,TNF-α,and IL-1βwere decreased(P<0.05).Conclusion Myricetin can inhibit the increased expression of inflammatory factors including NF-κB,IL-6,TNF-α,IL-1β,etc.induced by liver ischemia-reperfusion in mice,and alleviate liver damage.

关 键 词：杨梅素 肝脏 缺血再灌注损伤 炎症因子 核转录因子-ΚB 

分 类 号：R965[医药卫生—药理学]