BMP-6介导的铁蓄积在LPS诱导的急性肺损伤中的作用  

Role of BMP-6-mediated Iron Accumulation in LPS-induced Acute Lung Injury

作　　者：李卓越 李秦汉 洪怡然 付孝菊 肖驰闯 孙燕玲 林丽[1] LI Zhuo-yue;LI Qin-han;LIN Li(School of Clinical Medicine,Xianning Medical College,Hubei University of Science and Technology,Xianning Hubei 437100,China)

机构地区：[1]湖北科技学院医学部基础医学院,湖北咸宁437100

出　　处：《湖北科技学院学报（医学版）》2024年第6期498-501,共4页Journal of Hubei University of Science and Technology(Medical Sciences)

基　　金：湖北省大学生创新训练项目(S202110927022)。

摘　　要：目的探讨骨形态发生蛋白6(BMP-6)介导的铁蓄积在脂多糖(LPS)诱导的急性肺损伤中的作用。方法采用腹腔注射LPS建立急性肺损伤的动物模型,通过石蜡切片和苏木精-伊红(HE)染色法观察肺组织的病理学改变,实时荧光定量PCR(qPCR)检测BMP-6和铁调素(hepcidin)的mRNA表达量,免疫印迹试验(Western blot)分析BMP-6和hepcidin的蛋白表达水平,比色法测定血清和肺组织铁水平。结果大鼠注射LPS后,肺组织病理损伤明显,BMP-6和hepcidin的mRNA和蛋白表达水平下降,血清和肺组织铁水平升高。结论LPS可能通过下调BMP-6和hepcidin的表达,导致肺部铁水平升高,从而诱导急性肺损伤的发生。Objective To investigate the role of bone morphogenetic protein 6(BMP-6)-mediated iron accumulation in lipopolysaccharide(LPS)-induced acute lung injury.Methods The acute lung injury model was established by intraperitoneal injection of LPS.The pathological changes of lung tissue were observed by paraffin section and hematoxylin-eosin(HE)staining.The mRNA and protein expression levels of BMP-6 and hepcidin were detected by real-time fluorescence quantitative PCR(qPCR)and Western blot respectively,The iron levels in serum and lung tissues were determined by colorimetry.Results After LPS injection in rats,pathological damage in lung tissues increased,the mRNA and protein expression levels of BMP-6 and hepcidin decreased,and the iron levels in serum and lung tissues increased.Conclusion By down-regulating the expression of BMP-6 and hepcidin,LPS increases the level of iron in lung and induces the occurrence of acute lung injury.

关 键 词：急性肺损伤 骨形态发生蛋白6 铁调素 铁 

分 类 号：R965.1[医药卫生—药理学]