Sulfated liposome-based artificial cell membrane glycocalyx nanodecoys for coronavirus inactivation by membrane fusion  

作　　者：Xu Li Ningtao Cheng Danrong Shi Yutong Li Chen Li Miaojin Zhu Qiao Jin Zhigang Wu Linwei Zhu Yi He Hangping Yao Jian Ji 

机构地区：[1]MOE Key Laboratory of Macromolecule Synthesis and Functionalization,Department of Polymer Science and Engineering,Zhejiang University,Hangzhou,310027,China [2]School of Medicine,Zhejiang University,Hangzhou,310058,China [3]State Key Laboratory for Diagnosis and Treatment of Infectious Diseases,National Clinical Research Center for Infectious Diseases,Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases,The First Affiliated Hospital,School of Medicine,Zhejiang University,Hangzhou,310003,China [4]College of Chemical and Biological Engineering,Zhejiang University,Hangzhou,310027,China [5]Shanxi-Zheda Institute of Advanced Materials and Chemical Engineering,Taiyuan,030032,China

出　　处：《Bioactive Materials》2024年第3期1-13,共13页生物活性材料（英文）

基　　金：supported by the National Key Research&Development Program of China(No.2021YFC2301204);Shanxi-Zheda Institute of Advanced Materials and Chemical Engineering(2022SZ-TD010);the Fundamental Research Funds for the Central Universities(No.2022ZFJH003).We thank Lingyun Wu in the Center of Cryo-Electron Microscopy(CCEM),Zhejiang University for her technical assistance on Cryo-EM.

摘　　要：As a broad-spectrum antiviral nanoparticle,the cell membrane nanodecoy is a promising strategy for preventing viral infections.However,most of the cell membrane nanodecoys can only catch virus and cannot induce inactivation,which may bring about a considerably high risk of re-infection owing to the possible viral escape from the nanodecoys.To tackle this challenge,sulfated liposomes are employed to mimic the cell membrane glycocalyx for constructing an artificial cell membrane glycocalyx nanodecoy that exhibits excellent anti-coronavirus activity against HCoV-OC43,wild-type SARS-CoV-2,Alpha and Delta variant SARS-CoV-2 pseudovirus.In addition,this nanodecoy,loaded with surface sulfate groups as SARS-CoV-2 receptor arrays,can enhance the antiviral capability to virus inactivation through destroying the virus membrane structure and transfer the spike protein to postfusion conformation.Integrating bio-inspired recognition and inactivation of viruses in a single supramolecular entity,the artificial cell membrane nanodecoy opens a new avenue for the development of theranostic antiviral nanosystems,whose mass production is favored due to the facile engineering of sulfated liposomes.

关 键 词：Antiviral nanoparticle Cell membrane nanodecoy CORONAVIRUS Viral inactivation 

分 类 号：O62[理学—有机化学]