Cartilage-on-a-chip with magneto-mechanical transformation for osteoarthritis recruitment  

作　　者：Hao Liu Xiangyi Wu Rui Liu Weijun Wang Dagan Zhang Qing Jiang 

机构地区：[1]State Key Laboratory of Pharmaceutical Biotechnology,Department of Sports Medicine and Adult Reconstructive Surgery,Nanjing Drum Tower Hospital,The Affiliated Hospital of Nanjing University Medical School,321 Zhongshan Road,Nanjing,210008,Jiangsu,China [2]Department of Rheumatology and Immunology,Institute of Translational Medicine,The Affiliated Drum Tower Hospital of Nanjing University Medical School,Nanjing,210008,China

出　　处：《Bioactive Materials》2024年第3期61-68,共8页生物活性材料（英文）

基　　金：supported by the National Natural Science Foundation of China(82102511);the Natural Science Foundation of Jiangsu(BK20210021);Research Project of Jiangsu Province Health Committee (M2021031);the National Basic Research Program of China (2021YFA1201404);Major Project of NSFC (81991514);Jiangsu Provincial Key Medical Center Foundation, Jiangsu Provincial Medical Outstanding Talent Foundation, Jiangsu Provincial Medical Youth Talent Foundation and Jiangsu Provincial Key Medical Talent Foundation. Supported by fundings for Clinical Trials from the Affiliated Drum Tower Hospital, Medical School of Nanjing University (2022-LCYJ-PY- 05);the Fundamental Research Funds for the Central Universities (14380493, 14380494).

摘　　要：Osteoarthritis (OA) is a prevalent joint disease primarily induced by overstrain, leading to disability and significantly impacting patients’ quality of life. However, current OA studies lack an ideal in vitro model, which can recapitulate the high peripheral strain of the joint and precisely model the disease onset process. In this paper, we propose a novel cartilage-on-a-chip platform that incorporates a biohybrid hydrogel comprising Neodymium (NdFeB)/Poly-GelMA-HAMA remote magneto-control hydrogel film. This platform facilitates chondrocyte culture and stress loading, enabling the investigation of chondrocytes under various stress stimuli. The Neodymium (NdFeB)/Poly-GelMA-HAMA hydrogel film exhibits magneto-responsive shape-transition behavior, further dragging the chondrocytes cultured in hydrogels under magnetic stimulation. It was investigated that inflammation-related genes and proteins in chondrocytes are changed with mechanical stress stimulation in the cartilage-on-a-chip. Especially, MMP-13 and the proportion of collagen secretion are upregulated, showing a phenotype similar to that of real human osteoarthritis. Therefore, we believed that this cartilage-on-a-chip platform provides a desired in vitro model for osteoarthritis, which is of great significance in disease research and drug development.

关 键 词：Cartilage-on-a-chip Neodymium magnet GelMA-HAMA Mechanical loading 

分 类 号：O64[理学—物理化学]