Silk fibroin-based inks for in situ 3D printing using a double crosslinking process  

作　　者：Francesca Agostinacchio Vincent Fitzpatrick Sandra Dire David LKaplan Antonella Motta 

机构地区：[1]National Interuniversity Consortium of Material Science and Technology,Florence,Italy [2]BIOtech Research Center and European Institute of Excellence on Tissue Engineering and Regenerative Medicine,Department of Industrial Engineering,University of Trento,Trento,Italy [3]Materials Chemistry Group&“Klaus Müller”Magnetic Resonance Laboratory,Department of Industrial Engineering,University of Trento,Trento,Italy [4]Department of Biomedical Engineering,Tufts University,Medford,MA,02155,USA

出　　处：《Bioactive Materials》2024年第5期122-134,共13页生物活性材料（英文）

基　　金：funding from the Italian Ministry for Education,University,and Research(MIUR)within the program“Departments of Excellence”2018-2022(DII-UNITN);from the European Union’s Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant agreement no.101008041.;the NIH(P41EB027062)for support of this work.

摘　　要：The shortage of tissues and organs for transplantation is an urgent clinical concern.In situ 3D printing is an advanced 3D printing technique aimed at printing the new tissue or organ directly in the patient.The ink for this process is central to the outcomes,and must meet specific requirements such as rapid gelation,shape integrity,stability over time,and adhesion to surrounding healthy tissues.Among natural materials,silk fibroin exhibits fascinating properties that have made it widely studied in tissue engineering and regenerative medicine.However,further improvements in silk fibroin inks are needed to match the requirements for in situ 3D printing.In the present study,silk fibroin-based inks were developed for in situ applications by exploiting covalent crosslinking process consisting of a pre-photo-crosslinking prior to printing and in situ enzymatic crosslinking.Two different silk fibroin molecular weights were characterized and the synergistic effect of the covalent bonds with shear forces enhanced the shift in silk secondary structure towardβ-sheets,thus,rapid stabilization.These hydrogels exhibited good mechanical properties,stability over time,and resistance to enzymatic degradation over 14 days,with no significant changes over time in their secondary structure and swelling behavior.Additionally,adhesion to tissues in vitro was demonstrated.

关 键 词：Silk fibroin inks In situ 3D printing Covalent crosslinking Regenerative medicine Rapid gelation 

分 类 号：R318[医药卫生—生物医学工程]