An Injectable silk-based hydrogel as a novel biomineralization seedbed for critical-sized bone defect regeneration  

作　　者：Yuhui Zhu Hao Gu Jiawei Yang Anshuo Li Lingli Houh Mingliang Zhou Xinquan Jiang 

机构地区：[1]Department of Prosthodontics,Shanghai Ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine,No.639 Zhizaoju Road,Shanghai,200011,China [2]College of Stomatology,Shanghai Jiao Tong University,No.115 Jinzun Road,Shanghai,200125,China [3]National Center for Stomatology,No.639 Zhizaoju Road,Shanghai,200011,China [4]National Clinical Research Center for Oral Diseases,No.639 Zhizaoju Road,Shanghai,200011,China [5]Shanghai Key Laboratory of Stomatology,No.639 Zhizaoju Road,Shanghai,200011,China [6]Shanghai Research Institute of Stomatology,No.639 Zhizaoju Road,Shanghai,200011,China [7]Shanghai Engineering Research Center of Advanced Dental Technology and Materials,No.115 Jinzun Road,Shanghai 200125,China [8]Shanghai Institute of Precision Medicine,Shanghai Ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine,No.115 Jinzun Road,Shanghai,200125,China

出　　处：《Bioactive Materials》2024年第5期274-290,共17页生物活性材料（英文）

基　　金：supported by the National Natural Science Foundation of China(No.82130027,81921002,81991505,31900971);Cross Disciplinary Research Fund of Shanghai Ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine(JYJC202128);Moreover,we thank the Electron Microscopy center of Shanghai Institute of Precision Medicine,Shanghai Ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine,for their technical support and assistance in the electron microscopy.

摘　　要：The healing process of critical-sized bone defects urges for a suitable biomineralization environment. However, the unsatisfying repair outcome usually results from a disturbed intricate milieu and the lack of in situ mineralization resources. In this work, we have developed a composite hydrogel that mimics the natural bone healing processes and serves as a seedbed for bone regeneration. The oxidized silk fibroin and fibrin are incorporated as rigid geogrids, and amorphous calcium phosphate (ACP) and platelet-rich plasma serve as the fertilizers and loam, respectively. Encouragingly, the seedbed hydrogel demonstrates excellent mechanical and biomineralization properties as a stable scaffold and promotes vascularized bone regeneration in vivo. Additionally, the seedbed serves a succinate-like function via the PI3K-Akt signaling pathway and subsequently orchestrates the mitochondrial calcium uptake, further converting the exogenous ACP into endogenous ACP. Additionally, the seedbed hydrogel realizes the succession of calcium resources and promotes the evolution of the biotemplate from fibrin to collagen. Therefore, our work has established a novel silk-based hydrogel that functions as an in-situ biomineralization seedbed, providing a new insight for critical-sized bone defect regeneration.

关 键 词：Silk fibroin Bone regeneration HYDROGEL BIOMINERALIZATION Platelet-rich plasma 

分 类 号：R318[医药卫生—生物医学工程]