Versatile dopamine-functionalized hyaluronic acid-recombinant human collagen hydrogel promoting diabetic wound healing via inflammation control and vascularization tissue regeneration  

作　　者：Yong Wang Yuan Zhang Yun-Peng Yang Ming-Yuan Jin Sha Huang Ze-Ming Zhuang Tao Zhang Li-Li Cao Xiao-Ying Lin Jun Chen Yong-Zhong Du Jian Chen Wei-Qiang Tan 

机构地区：[1]Department of Plastic Surgery,Sir Run Run Shaw Hospital,Zhejiang University School of Medicine,3 East Qingchun Road,Hangzhou,310016,China [2]Department of Ultrasound in Medicine,The Fourth Affiliated Hospital,Zhejiang University School of Medicine,Yiwu,322000,China [3]Institute of Pharmaceutics,College of Pharmaceutical Sciences,Zhejiang University,866 Yu-Hang-Tang Road,Hangzhou,310058,China [4]MOE Key Laboratory of Biosystems Homeostasis&Protection,College of Life Sciences,Zhejiang University,Hangzhou,310058,China

出　　处：《Bioactive Materials》2024年第5期330-345,共16页生物活性材料（英文）

基　　金：supported by grants from National Natural Science Foundation of China No.82172206;Zhejiang Provincial Medical and Healthy Science Foundation of China(No.2023RC183 and 2024KY110).

摘　　要：The management of chronic wounds in diabetes remains challenging due to the complexity of impaired wound healing,delayed healing,susceptibility to infection,and elevated risk of reopening,highlighting the need for effective chronic wound management with innovative approaches such as multifunctional hydrogels.Here,we have produced HA-DA@rhCol hydrogels consisting of dopamine-modified hyaluronic acid and recombinant human collagen type-III(rhCol)by oxidative coupling of the catechol group using the H_(2)O_(2)/HRP catalytic system.The post-reactive hydrogel has a good porous structure,swelling rate,reasonable degradation,rheological and mechanical properties,and the catechol group and dopamine impart to the hydrogel tissue adhesiveness,antioxidant capacity,and excellent photothermal effects leading to superior in vitro antimicrobial activity.In addition,the ability of rhCol to confer hydrogels to promote angiogenesis and wound repair has also been investigated.Cytotoxicity and hemolysis tests demonstrated the good biocompatibility of the hydrogel.Wound closure,collagen deposition and immunohistochemical examination confirmed the ability of the hydrogel to promote diabetic wound healing.In summary,the adhesive hemostatic antioxidative hydrogel with rhCol to promote wound healing in diabetic rat is an excellent chronic wound dressing.

关 键 词：Hyaluronic acid hydrogel Recombinant human collagen Antioxidant and anti-inflammation Antibacterial Diabetic wound 

分 类 号：O64[理学—物理化学]