Fabrication of fully aligned self-assembled cell-laden collagen filaments for tissue engineering via a hybrid bioprinting process  

作　　者：JuYeon Kim Hyeongjin Lee Gyudo Lee Dongryeol Ryu GeunHyung Kim 

机构地区：[1]Department of Precision Medicine,Sungkyunkwan University School of Medicine(SKKU-SOM),Suwon,16419,Republic of Korea [2]Department of Biotechnology and Bioinformatics,Korea University,Sejong,Republic of Korea [3]Department of Biomedical Science and Engineering,Gwangju Institute of Science and Technology,Gwangju,Republic of Korea

出　　处：《Bioactive Materials》2024年第6期14-29,共16页生物活性材料（英文）

基　　金：supported by the“Korea National Institute of Health”research project(2022ER130500);supported by a grant from the Ministry of Trade,Industry&Energy(MOTIE,Korea)under Industrial Technology Innovation Program(20009652:Technology on commercialization and materials of Bioabsorbable Hydroxyapatite less than 1μm in size).

摘　　要：Cell-laden structures play a pivotal role in various tissue engineering applications,particularly in tissue restoration.Interactions between cells within bioprinted structures are crucial for successful tissue development and regulation of stem cell fate through intricate cell-to-cell signaling pathways.In this study,we developed a new technique that combines polyethylene glycol(PEG)-infused submerged bioprinting with a stretching procedure.This approach facilitated the generation of fully aligned collagen structures consisting of myoblasts and a low concentration(2 wt%)of collagen to efficiently encourage muscle tissue regeneration.By adjusting several processing parameters,we obtained biologically safe and mechanically stable cell-laden collagen filaments with uniaxial alignment.Notably,the cell filaments exhibited markedly elevated cellular activities compared to those exhibited by conventional bioprinted filaments,even at similar cell densities.Moreover,when we implanted structures containing adipose stem cells into mice,we observed a significantly increased level of myogenesis compared to that in normally bioprinted struts.Thus,this promising approach has the potential to revolutionize tissue engineering by fostering enhanced cellular interactions and promoting improved outcomes in regenerative medicine.

关 键 词：Polyethylene glycol DEHYDRATION COLLAGEN Bioink Submerged bioprinting Muscle 

分 类 号：R318[医药卫生—生物医学工程]