Advanced PEG-tyramine biomaterial ink for precision engineering of perfusable and flexible small-diameter vascular constructs via coaxial printing  

作　　者：Julia Simińska-Stanny Lise Nicolas Adam Chafai Hafez Jafari Maryam Hajiabbas Gianina Dodi Ioannis Gardikiotis Christine Delporte Lei Nie Daria Podstawczyk Amin Shavandi 

机构地区：[1]UniversitéLibre de Bruxelles(ULB),École polytechnique de Bruxelles,3BIO-BioMatter,Avenue F.D.Roosevelt,50-CP 165/61,1050,Brussels,Belgium [2]European School of Materials Science and Engineering,University of Lorraine,Nancy,France [3]UniversitéLibre de Bruxelles(ULB),Micro-milli Platform,Avenue F.D.Roosevelt,50-CP 165/67,1050,Brussels,Belgium [4]Faculty of Medical Bioengineering,Grigore T.Popa,University of Medicine and Pharmacy of Iasi,Romania [5]Advanced Research and Development Center for Experimental Medicine,Grigore T.Popa,University of Medicine and Pharmacy of Iasi,Romania [6]UniversitéLibre de Bruxelles(ULB),Facultéde Médecine,Campus Erasme-CP 611,Laboratory of Pathophysiological and Nutritional Biochemistry,Route de Lennik,808,1070,Bruxelles,Belgium [7]College of Life Science,Xinyang Normal University,Xinyang,China [8]Department of Process Engineering and Technology of Polymer and Carbon Materials,Faculty of Chemistry,Wroclaw University of Science and Technology,Norwida 4/6,50-373,Wroclaw,Poland

出　　处：《Bioactive Materials》2024年第6期168-184,共17页生物活性材料（英文）

基　　金：supported by an Aspirant fellowship from the Fonds National de la Recherche Scientific de Belgique(FNRS)(grant number 46599,2022 awarded to Julia Siminska-Stanny);A.S.ac-knowledges FNRS for providing a Research Credit(CDR)with grant number J.0188.24.M.H.acknowledges the financial support of Wallo-nia Brussels Internation(WBI).

摘　　要：Vascularization is crucial for providing nutrients and oxygen to cells while removing waste.Despite advances in 3D-bioprinting,the fabrication of structures with void spaces and channels remains challenging.This study presents a novel approach to create robust yet flexible and permeable small(600-1300μm)artificial vessels in a single processing step using 3D coaxial extrusion printing of a biomaterial ink,based on tyramine-modified polyethylene glycol(PEG-Tyr).We combined the gelatin biocompatibility/activity,robustness of PEG-Tyr and alginate with the shear-thinning properties of methylcellulose(MC)in a new biomaterial ink for the fabrication of bioinspired vessels.Chemical characterization using NMR and FTIR spectroscopy confirmed the successful modification of PEG with Tyr and rheo-logical characterization indicated that the addition of PEG-Tyr decreased the viscosity of the ink.Enzyme-mediated crosslinking of PEG-Tyr allowed the formation of covalent crosslinks within the hydrogel chains,ensuring its stability.PEG-Tyr units improved the mechanical properties of the material,resulting in stretchable and elastic constructs without compromising cell viability and adhesion.The printed vessel structures displayed uniform wall thickness,shape retention,improved elasticity,permeability,and colonization by endothelial-derived-EA.hy926 cells.The chorioallantoic membrane(CAM)and in vivo assays demonstrated the hydrogel’s ability to support neoangiogenesis.The hydrogel material with PEG-Tyr modification holds promise for vascular tissue engineering applications,providing a flexible,biocompatible,and functional platform for the fabrication of vascular structures.

关 键 词：PRINTING COAXIAL removing 

分 类 号：R318.08[医药卫生—生物医学工程]