Antibiofilm and antithrombotic hydrogel coating based on superhydrophilic zwitterionic carboxymethyl chitosan for blood-contacting devices  

作　　者：Dong Uk Lee Mukhammad Kayumov Junghun Park Se Kye Park Yeongkwon Kang Yejin Ahn Woojin Kim Seung Hwa Yoo Jun-Kyu Park Bong-Gi Kim Yong Suk Oh In-Seok Jeong Dong Yun Choi 

机构地区：[1]Biomedical Manufacturing Technology Center,Korea Institute of Industrial Technology,Yeongcheon,38822,Republic of Korea [2]Department of Thoracic and Cardiovascular Surgery,Chonnam National University Hospital and Medical School,Gwangju,61469,Republic of Korea [3]Research Institute of Advanced Materials,Seoul National University,Seoul,08826,Republic of Korea [4]Department of Organic and Nano System Engineering,Konkuk University,Seoul,05029,Republic of Korea [5]Department of Quantum System Engineering,Jeonbuk National University,Jeonju-si,54896,Republic of Korea [6]IMT Inc.,Gwangju,61086,Republic of Korea [7]Department of Mechanical Engineering,Changwon National University,Changwon,51140,Republic of Korea

出　　处：《Bioactive Materials》2024年第4期112-124,共13页生物活性材料（英文）

基　　金：supported by the Korea Evaluation Institute of Industrial Technology(KEIT)grant funded by the South Korea government(MOTIE)(No.1415187426,RS-2023-00238181);supported by the institutional program funded by the Korea Institute of Industrial Technology(JA230007);the National Research Foundation of Korea(NRF)grant funded by the South Korea government(MSIT)(No.2022R1F1A1074255,No.RS-2022-00144435).

摘　　要：Blood-contacting devices must be designed to minimize the risk of bloodstream-associated infections,thrombosis,and intimal lesions caused by surface friction.However,achieving effective prevention of both bloodstream-associated infections and thrombosis poses a challenge due to the conflicting nature of antibacterial and antithrombotic activities,specifically regarding electrostatic interactions.This study introduced a novel biocompatible hydrogel of sodium alginate and zwitterionic carboxymethyl chitosan(ZW@CMC)with antibacterial and antithrombotic activities for use in catheters.The ZW@CMC hydrogel demonstrates a superhydrophilic surface and good hygroscopic properties,which facilitate the formation of a stable hydration layer with low friction.The zwitterionic-functionalized CMC incorporates an additional negative sulfone group and increased negative charge density in the carboxyl group.This augmentation enhances electrostatic repulsion and facilitates the formation of hydration layer.This leads to exceptional prevention of blood clotting factor adhesion and inhibition of biofilm formation.Subsequently,the ZW@CMC hydrogel exhibited biocompatibility with tests of in vitro cytotoxicity,hemolysis,and catheter friction.Furthermore,in vivo tests of antithrombotic and systemic inflammation models with catheterization indicated that ZW@CMC has significant advantages for practical applications in cardiovascular-related and sepsis treatment.This study opens a new avenue for the development of chitosan-based multifunctional hydrogel for applications in blood-contacting devices.

关 键 词：ZWITTERIONIC Hydrogel Blood-clotting Antimicrobial Sepsis 

分 类 号：R318.08[医药卫生—生物医学工程] TQ427.26[医药卫生—基础医学]