A parathyroid hormone related supramolecular peptide for multi-functionalized osteoregeneration  

作　　者：Zhuowen Hao Qinyu Feng Yi Wang Ying Wang Hanke Li Yingkun Hu Tianhong Chen Junwu Wang Renxin Chen Xuan Lv Zhiqiang Yang Jiayao Chen Xiaodong Guo Jingfeng Li 

机构地区：[1]Department of Orthopedics,Zhongnan Hospital of Wuhan University,Wuhan,430071,China [2]Department of Obstetrics and Gynecology,Renmin Hospital of Wuhan University,Wuhan,430060,China [3]Department of Orthopedics,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,430022,China

出　　处：《Bioactive Materials》2024年第4期181-203,共23页生物活性材料（英文）

基　　金：supported by the National Natural Science Foundation of China(No.82372405,No.81871752);the Fundamental Research Funds for the Central Universities(NO.2042023kf0199);the Key Research and Development Program of Hubei Province(No:2022BCA052);the Key Research and Development Program of Wuhan City(No.2023020402010591);the Translational Medicine and Interdisciplinary Research Joint Fund of Zhongnan Hospital of Wuhan University(No.ZNJC202014).

摘　　要：Supramolecular peptide nanofiber hydrogels are emerging biomaterials for tissue engineering,but it is difficult to fabricate multi-functional systems by simply mixing several short-motif-modified supramolecular peptides because relatively abundant motifs generally hinder nanofiber cross-linking or the formation of long nanofiber.Coupling bioactive factors to the assembling backbone is an ideal strategy to design multi-functional supramolecular peptides in spite of challenging synthesis and purification.Herein,a multi-functional supramolecular peptide,P1R16,is developed by coupling a bioactive factor,parathyroid hormone related peptide 1(PTHrP-1),to the basic supramolecular peptide RADA16-I via solid-phase synthesis.It is found that P1R16 self-assembles into long nanofibers and co-assembles with RADA16-I to form nanofiber hydrogels,thus coupling PTHrP-1 to hydrogel matrix.P1R16 nanofiber retains osteoinductive activity in a dose-dependent manner,and P1R16/RADA16-I nanofiber hydrogels promote osteogenesis,angiogenesis and osteoclastogenesis in vitro and induce multi-functionalized osteoregeneration by intramembranous ossification and bone remodeling in vivo when loaded to collagen(Col)scaffolds.Abundant red blood marrow formation,ideal osteointegration and adapted degradation are observed in the 50%P1R16/Col scaffold group.Therefore,this study provides a promising strategy to develop multi-functional supramolecular peptides and a new method to topically administrate parathyroid hormone or parathyroid hormone related peptides for non-healing bone defects.

关 键 词：Supramolecular peptide nanofiber hydrogels Parathyroid hormone SELF-ASSEMBLY Co-assembly Bone tissue engineering 

分 类 号：R318.08[医药卫生—生物医学工程] TB383[医药卫生—基础医学]