Construction of tissue-engineered vascular grafts with enhanced patency by integrating heparin,cell-adhesive peptide,and carbon monoxide nanogenerators into acellular blood vessels  

作　　者：Yonghong Fan Juan Pei Yinhua Qin Huifang Du Xiaohang Qu Wenya Li Boyue Huang Ju Tan Yong Liu Gang Li Ming Ke Youqian Xu Chuhong Zhu 

机构地区：[1]Department of Anatomy,Engineering Research Center for Organ Intelligent Biological Manufacturing of Chongqing,Key Lab for Biomechanics and Tissue Engineering of Chongqing,Third Military Medical University,Chongqing,400038,China [2]Laboratory of Basic Medicine,The General Hospital of Western Theater Command,Chengdu,610083,China [3]Engineering Research Center of Tissue and Organ Regeneration and Manufacturing,Ministry of Education,Chongqing,400038,China [4]State Key Laboratory of Trauma and Chemical Poisoning,Chongqing,400038,China [5]Department of Plastic and Aesthetic Surgery,Southwest Hospital,Third Military Medical University,Chongqing,400038,China

出　　处：《Bioactive Materials》2024年第4期221-236,共16页生物活性材料（英文）

基　　金：supported by the Key Projects of the National Natural Science Foundation of China(81830055);National Natural Science Foundation of China(82001966);Outstanding Scientist Project of Chongqing(cstc2022ycjh-bgzxm0186);Special Funding for Postdoctoral Research of Chongqing(2021XM1020).

摘　　要：Small-diameter tissue-engineered vascular grafts(sdTEVGs)have garnered significant attention as a potential treatment modality for vascular bypass grafting and replacement therapy.However,the intimal hyperplasia and thrombosis are two major complications that impair graft patency during transplantation.To address this issue,we fabricated the covalent-organic framework(COF)-based carbon monoxide(CO)nanogenerator-and co-immobilized with LXW-7 peptide and heparin to establish a multifunctional surface on TEVGs constructed from acellular blood vessels for preventing thrombosis and stenosis.The cell-adhesive peptide LXW-7 could capture endothelial-forming cells(EFCs)to promote endothelialization,while the antithrombotic molecule heparin prevented thrombus formation.The reactive oxygen species(ROS)-triggered CO release suppressed the adhesion and activation of macrophages,leading to the reduction of ROS and inflammatory factors.As a result,the endothelial-to-mesenchymal transition(EndMT)triggered by inflammation was restricted,facilitating the maintenance of the homeostasis of the neo-endothelium and preventing pathological remodeling in TEVGs.When transplanted in vivo,these vascular grafts exhibited negligible intimal hyperplasia and remained patent for 3 months.This achievement provided a novel approach for constructing antithrombotic and anti-hyperplastic TEVGs.

关 键 词：Tissue engineered vascular grafts Carbon monoxide Heparin Endothelial-to-mesenchymal transition 

分 类 号：R318[医药卫生—生物医学工程]