2-N,6-O sulfated chitosan evokes periosteal stem cells for bone regeneration  

作　　者：Luli Ji Yuanman Yu Fuwei Zhu Dongao Huang Xiaogang Wang Jing Wang Changsheng Liu 

机构地区：[1]Key Laboratory for Ultrafine Materials of Ministry of Education,East China University of Science and Technology,Shanghai,200237,PR China [2]The State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,Shanghai,200237,PR China [3]Engineering Research Center for Biomedical Materials of the Ministry of Education,East China University of Science and Technology,Shanghai,200237,PR China [4]Frontiers Science Center for Materiobiology and Dynamic Chemistry,East China University of Science and Technology,Shanghai,200237,PR China

出　　处：《Bioactive Materials》2024年第4期282-297,共16页生物活性材料（英文）

基　　金：supported by National Natural Science Foundation of China for Innovative Research Groups(No.51621002);the National Natural Science Foundation of China(No.31870953);supported by the 111 Project(B14018).

摘　　要：Musculoskeletal injuries and bone defects represent a significant clinical challenge,necessitating innovative approaches for effective bone tissue regeneration.In this study,we investigated the potential of harnessing periosteal stem cells(PSCs)and glycosaminoglycan(GAG)-mimicking materials for in situ bone regeneration.Our findings demonstrated that the introduction of 2-N,6-O sulfated chitosan(26SCS),a GAG-like polysaccharide,enriched PSCs and promoted robust osteogenesis at the defect area.Mechanistically,26SCS amplifies the biological effect of endogenous platelet-derived growth factor-BB(PDGF-BB)through enhancing the interaction between PDGF-BB and its receptor PDGFRβabundantly expressed on PSCs,resulting in strengthened PSC proliferation and osteogenic differentiation.As a result,26SCS effectively improved bone defect repair,even in an osteoporotic mouse model with lowered PDGF-BB level and diminished regenerative potential.Our findings suggested the significant potential of GAG-like biomaterials in regulating PSC behavior,which holds great promise for addressing osteoporotic bone defect repair in future applications.

关 键 词：2-N 6-O sulfated chitosan Periosteal stem cells PDGF-BB Bone regeneration 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]