Integrating coaxial electrospinning and 3D printing technologies for the development of biphasic porous scaffolds enabling spatiotemporal control in tumor ablation and osteochondral regeneration  

作　　者：Wenbao He Chunlin Li Shitong Zhao Zhendong Li Jing Wu Junjun Li Haichao Zhou Yunfeng Yang Yong Xu Huitang Xia 

机构地区：[1]Department of Thoracic Surgery,Shanghai Pulmonary Hospital,School of Medicine,Tongji University,Shanghai,China [2]Department of Orthopedics,Shanghai Tongji Hospital,School of Medicine,Tongji University,Shanghai,China [3]Department of Plastic Surgery,The First Affiliated Hospital of Shandong First Medical University&Shandong Provincial Qianfoshan Hospital,Jinan,Shandong,250014,PR China [4]Jinan Clinical Research Centre for Tissue Engineering Skin Regeneration and Wound Repair,The First Affiliated Hospital of Shandong First Medical University,Jinan,China [5]Department of Orthopaedics,Qilu Hospital of Shandong University,Shandong University Centre for Orthopaedics,Advanced Medical Research Institute,Cheeloo College of Medicine,Shandong University,Jinan,China [6]Department of Neurosurgery,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,China [7]Department of Orthopaedics,Ruijin Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai,China

出　　处：《Bioactive Materials》2024年第4期338-353,共16页生物活性材料（英文）

基　　金：supported by the National Natural Science Foundation of China(82302395 and 82001979);Natural Science Foundation of Shanghai(22YF1437400);Young Elite Scientists Sponsorship Program by CAST(2023QNRC001);Shandong Provincial Natural Science Foundation(Major Basic Research Program,ZR2019ZD38);Taishan Scholar Program of Shandong Province(To Huitang Xia,202211333);Natural Science Foundation of Shandong Province(ZR2020MH381);Academic Promotion Program of Shandong First Medical University(2019LJ005);Shandong First Medical University Culture Foundation(202201-09);Social Science Planning and Research Project of Shandong Province(21CTQJ08).

摘　　要：The osteochondral defects(OCDs)resulting from the treatment of giant cell tumors of bone(GCTB)often present two challenges for clinicians:tumor residue leading to local recurrence and non-healing of OCDs.Therefore,this study focuses on developing a double-layer PGPC-PGPH scaffold using shell-core structure nanofibers to achieve“spatiotemporal control”for treating OCDs caused by GCTB.It addresses two key challenges:eliminating tumor residue after local excision and stimulating osteochondral regeneration in non-healing OCD cases.With a shell layer of protoporphyrin IX(PpIX)/gelatin(GT)and inner cores containing chondroitin sulfate(CS)/poly(lactic-co-glycolic acid)(PLGA)or hydroxyapatite(HA)/PLGA,coaxial electrospinning technology was used to create shell-core structured PpIX/GT-CS/PLGA and PpIX/GT-HA/PLGA nanofibers.These nanofibers were shattered into nano-scaled short fibers,and then combined with polyethylene oxide and hyaluronan to formulate distinct 3D printing inks.The upper layer consists of PpIX/GT-CS/PLGA ink,and the lower layer is made from PpIX/GT-HA/PLGA ink,allowing for the creation of a double-layer PGPC-PGPH scaffold using 3D printing technique.After GCTB lesion removal,the PGPC-PGPH scaffold is surgically implanted into the OCDs.The sonosensitizer PpIX in the shell layer undergoes sonodynamic therapy to selectively damage GCTB tissue,effectively eradicating residual tumors.Subsequently,the thermal effect of sonodynamic therapy accelerates the shell degradation and release of CS and HA within the core layer,promoting stem cell differentiation into cartilage and bone tissues at the OCD site in the correct anatomical position.This innovative scaffold provides temporal control for anti-tumor treatment followed by tissue repair and spatial control for precise osteochondral regeneration.

关 键 词：Spatiotemporal control Giant cell tumors of bone Shell-core structure Double-layered scaffold Osteochondral regeneration 

分 类 号：R318[医药卫生—生物医学工程] R73[医药卫生—基础医学]