基于电感耦合等离子体质谱法的天竺黄中无机元素的分析  

Analysis of inorganic elements in Bambusae Concretio Silicea based on inductively coupled plasma mass spectrometry

作　　者：余春霞 孔祥河 任广喜 徐裕彬 姜丹[2] 刘春生[2] YU Chun-xia;KONG Xiang-he;REN Guang-xi;XU Yu-bin;JIANG Dan;LIU Chun-sheng(Henan Provincial Center for Drug Evaluation and Inspection,Zhengzhou 450000,China;Beijing University of Chinese Medicine,Beijing 102488,China;Hebei Jujing Pharmaceutical Co.,Ltd.,Anguo 071200,China)

机构地区：[1]河南省药品审评查验中心,河南郑州450000 [2]北京中医药大学,北京102488 [3]河北橘井药业有限公司,河北安国071200

出　　处：《中国中药杂志》2024年第20期5497-5504,共8页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(82173921);中央高校横向课题(2020110031001469)。

摘　　要：运用电感耦合等离子体质谱(ICP-MS)法,测定天竺黄中的25种无机元素含量,并根据元素含量,建立元素指纹图谱,同时运用SPSS 20.0和SIMCA 14.1进行化学计量学分析。结果发现,在天竺黄中共测得25种元素,分别为硼(B)、钠(Na)、镁(Mg)、铝(Al)、钾(K)、钙(Ca)、钪(Sc)、钒(V)、铬(Cr)、锰(Mn)、铁(Fe)、钴(Co)、镍(Ni)、铜(Cu)、锌(Zn)、镓(Ga)、砷(As)、硒(Se)、锆(Zr)、钼(Mo)、镉(Cd)、铟(In)、钡(Ba)、铅(Pb)和铋(Bi),可定量的为13种元素,平均含量排名前三且由高到低依次是K>Fe>Na,其中B、Na、Al、Ca、Fe和Ba这6种元素在不同产地批次天竺黄中含量差别较大。通过相关性分析,发现Ca和Mg之间相关系数最高,其次是Mn和Zn,均呈现显著正相关关系,并且推测Ca和Mg、Mn和Zn分别存在协同增量的关系;由主成分分析可得,Fe、As、Ni、Cu、Mn、Na、Mg、Ca、Al、Ba和B元素是天竺黄的主要元素;由正交偏最小二乘法判别分析可得,Cu、As、Mn是3个产地的共同差异元素,表明通过对Cu、As、Mn 3种元素的定量鉴别,可使3个产地批次的天竺黄简单区分。该研究通过对不同产地批次天竺黄元素种类和含量进行测定分析,以期为天竺黄药效物质基础及调控机制的探究提供理论支撑,为天竺黄质量评价、临床用药安全及资源开发利用奠定基础。Inductively coupled plasma mass spectrometry was employed to determine the content of 25 inorganic elements in Bambusae Concretio Silicea,and the elemental fingerprint was established according to the element content.SPSS 20.0 and SIMCA 14.1 were used for chemometric analysis.A total of 25 elements were detected in Bambusae Concretio Silicea,including boron(B),sodium(Na),magnesium(Mg),aluminum(Al),potassium(K),calcium(Ca),scandium(Sc),vanadium(V),chromium(Cr),manganese(Mn),iron(Fe),cobalt(Co),nickel(Ni),copper(Cu),zinc(Zn),gallium(Ga),arsenic(As),selenium(Se),zirconium(Zr),molybdenum(Mo),cadmium(Cd),indium(In),barium(Ba),plumbum(Pb),and bismuth(Bi),among which 13 elements were quantitative.The top three elements in terms of average content were K>Fe>Na,and the content of B,Na,Al,Ca,Fe,and Ba varied in different batches of Bambusae Concretio Silicea samples from different origins.The correlation coefficient between Ca and Mg was the highest,followed by that between Mn and Zn,both of which showed significant positive correlations.Therefore,it was hypothesized that there was a synergistic incremental relationship between Ca and Mg as well as between Mn and Zn.According to the results of principal component analysis,Fe,As,Ni,Cu,Mn,Na,Mg,Ca,Al,Ba,and B were the main elements of Bambusae Concretio Silicea.According to the results of orthogonal partial least squares-discriminant analysis,Cu,As,and Mn were the common differential elements of three origins,which suggested that the quantitative identification of Cu,As,and Mn could roughly distinguish the three batches of Bambusae Concretio Silicea samples.This study analyzed the elements and their content in different batches of Bambusae Concretio Silicea samples,aiming to provide theoretical support for exploring the pharmacodynamic material basis and mechanism of Bambusae Concretio Silicea and lay a foundation for the quality evaluation,safe application,and resource development and utilization of Bambusae Concretio Silicea.

关 键 词：天竺黄 电感耦合等离子体质谱(ICP-MS) 无机元素 差异 

分 类 号：R284.1[医药卫生—中药学]