雌激素及其受体在非侵袭性真菌性鼻-鼻窦炎发病过程中的作用及其机制  

The Effect and Mechanism of Estrogen and Estrogen Receptors in the Pathogenesis of Non-Invasive Fungal Rhinosinusitis

作　　者：杨传梅 苏雅妃 沈潞华 张虹 王腾 季思宇 肖志勇 YANG Chuanmei;SU Yafei;SHEN Luhua;ZHANG Hong;WANG Teng;JI Siyu;XIAO Zhiyong(Department of Otolaryngology,Fuzhou Second General Hospital,Fuzhou 350007,China)

机构地区：[1]福州市第二总医院耳鼻咽喉科,福建福州350007

出　　处：《中山大学学报（医学科学版）》2024年第6期1017-1025,共9页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：福建省自然科学基金(2020J011190)。

摘　　要：【目的】探索雌激素及雌激素受体(ER)对非侵袭性真菌性鼻-鼻窦炎(NIFS)发病过程及其调控机制。【方法】选取2020年11月至2023年11月期间福州市第二总医院符合纳入标准的60例NIFS患者标本归为NIFS组,30位健康志愿者的样本归为空白对照组。分别收集各组标本,HE染色分别检测各组中嗜酸性粒细胞,和肥大细胞数量,免疫组化和免疫荧光检测ER表达情况,qPCR测定NF-κB、IKK和MASPIN的mRNA表达水平,免疫组化和免疫印迹检测NF-κB、IKK和MASPIN的蛋白表达水平。【结果】NIFS组中嗜酸性粒细胞和肥大细胞数量分别较对照组增加,组间差异有统计学意义(分别为P<0.01和P<0.05)。NIFS组中ER评分较对照组增加,组间差异有统计学意义(P<0.05),且NIFS组中平均高密度值较对照组增加,组间差异有统计学意义(P<0.01)。NIFS组的NF-κB、核因子κB抑制因子激酶(IKK)和乳腺丝氨酸蛋白酶抑制(MASPIN)较对照组增加,组间差异有统计学意义(分别为P<0.01、P<0.05和P<0.05),NIFS组的NF-κB、IKK和MASPIN mRNA的表达水平分别较对照组增加(P<0.01),且NIFS组中NF-κB、IKK和MASPIN蛋白的表达水平增加,组间差异有统计学意义(P<0.01、P<0.01和P<0.05)。【结论】我们的研究结果显示,ER、NF-κB、IKK和MASPIN表达水平显著升高,可能表明ER阳性患者嗜酸性粒细胞和肥大细胞浸润显著增加,提示雌激素及其受体参与了NIFS的发病机制。【Objective】To explore the role of estrogen and estrogen receptor(ER)in the pathogenesis and regulation of non-invasive fungal rhinosinusitis(NIFR).【Methods】Totally 60 patients with NIFS who met the inclusion criteria in Fuzhou Second General Hospital from November 2020 to November 2023 were selected as the NIFS group,while 30 healthy volunteers were recruited as the blank control group.Samples of each group were collected.The number of eosinophils and mast cells in each group were detected by HE staining;ER expression was detected by immunohistochemistry and immunofluorescence;mRNA expression levels of NF-κB,IKK and MASPIN were detected by qPCR;and protein expression levels of NF-κB,IKK and MASPIN were detected by immunohistochemistry and Western blot.【Results】In the NIFS group,the counts of eosinophils and mast cells were significantly increased respectively,compared with those in the control group,and the inter-group differences are statistically significant(P<0.01 and P<0.05,respectively).The Estrogen Receptor(ER)score in the NIFS group was significantly increased compared with that in the control group,and the inter-group differences are statistically significant(P<0.05).Additionally,the average high density value in the NIFS group was significantly increased compared with that in the control group,and the inter-group differences are statistically significant(P<0.01).The expression levels of NF-κB,IKK,and MASPIN in the NIFS group were significantly increased respectively,compared with those in the control group,and the inter-group differences are statistically significant(P<0.01,P<0.05,and P<0.01,respectively).The mRNA expression levels of NF-κB,IKK,and MASPIN in the NIFS group were significantly increased,respectively,compared with those in the control group(P<0.01).Furthermore,the protein expression levels of NFκB,IKK,and MASPIN in the NIFS group were increased,respectively,and the inter-group differences are statistically significant(P<0.01,P<0.01,and P<0.05,respectively).【Conclusion】O

关 键 词：非侵袭型真菌性鼻-鼻窦炎 雌激素受体 嗜酸性粒细胞 肥大细胞 核因子ΚB 核因子κB抑制因子激酶 乳腺丝氨酸蛋白酶抑制剂 

分 类 号：R765.9[医药卫生—耳鼻咽喉科]