基于数据挖掘和网络药理学研究《温病条辨》中人参方药治疗温病的用药规律  

Study on the rule of using Panax Ginseng prescription to treat warm diseases in Detailed Analysis of Warm Diseases based on data mining and network pharmacology

作　　者：刘冬雪 林春盛 王佳婧 孙兴华[2] 逄静 王曼玉 李佼阳 张福利[1] Liu Dongxue;Lin Chunsheng;Wang Jiajing;Sun Xinghua;Pang Jing;Wang Manyu;Li Jiaoyang;Zhang Fuli(Heilongjiang University of Traditional Chinese Medicine,Harbin 150040,China;The Second Affiliated Hospital of Heilongjiang University of Traditional Chinese Medicine,Harbin 150001,China)

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040 [2]黑龙江中医药大学附属第二医院,黑龙江哈尔滨150001

出　　处：《实用药物与临床》2024年第11期815-823,共9页Practical Pharmacy and Clinical Remedies

基　　金：黑龙江省中医药管理局项目(ZYW2023-020)。

摘　　要：目的探索吴鞠通在《温病条辨》中运用人参方药治疗温病的核心药物组合及配伍规律,以期为后续研究与应用提供参考依据。方法以“人参”为关键词,整理选取《温病条辨》中含有人参的方剂并记录。运用中医传承计算平台(TCMICS)对所提取的中药数据进行频次统计、性味归经统计、药物功效分析、聚类分析及关联规则分析,并总结出核心药物组合。运用中药系统药理学数据库与分析平台(TCMSP)、本草组鉴数据库(HERB)、PubChem数据库、Swiss Target Prediction数据库分析治疗温病药物的活性成分及靶点,使用Cytoscape软件构建核心药物-活性成分-靶点之间的网络图。通过DAVID数据库分析其相互作用关系及相关通路。结果共筛选出目标方剂43首。用药药性以温为主,其次为寒、平。药味以甘为主,其次为苦、辛。归经主要归脾、肺、心、肾经。中药功效频次最高的为补虚类,其次为收涩类、清热类。与人参组合的核心药物4味,频次由高到低分别为麦冬、炙甘草、当归、阿胶。药物组合中的活性成分共116个、靶点949个。ESR1、ESR2、CYP19A1、PTPN1等为较重要的靶点。核心药物活性成分主要涉及蛋白质丝氨酸/苏氨酸激酶活性、蛋白酪氨酸激酶活性、核受体活性、配体激活的转录因子活性等分子功能。潜在的作用靶点主要位于膜筏、膜微区、膜区等位置。共涉及199条通路,主要有神经活性配体-受体相互作用、钙信号、cAMP信号等通路。结论《温病条辨》中43首使用人参治疗温病的方剂药性以温为主,使用补虚类药物频次最高,其中人参-麦冬-炙甘草-当归-阿胶为核心药物组合,通过核心药物的主要活性成分及其潜在靶点,可得出人参治疗温病注重阴液与脾肺之气在温病逐渐变化中的影响,以调节人体阴阳与脏腑精气血津的虚实,达到扶正祛邪的目的。Objective To explore core drug combinations and medication regularity in using Panax Ginsengprescription to treat warm diseases in Wu Jutong's Detailed Analysis of Warm Diseases,aiming to provide reference forfuture study and application.Methods Taking Panax Ginseng as the key words,the prescriptions containing PanaxGinseng in Detailed Analysis of Warm Diseases were sorted out,selected and recorded.The data concerning frequencystatistics,statistics of channel tropism,drug efficacy analysis,drug clustering analysis and association rule analysis ofTCM(traditional Chinese medicine)were carried out by using the TCMICS,and the core drug combinations weresummarized.The active components and targets of the drugs for the treatment of warm diseases were analyzed by usingthe Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP),HERB database,PubChem database and Swiss Target Prediction database.Cytoscape software was used to map the core drug-activecomponent-target protein network.DAVID database was used to analyze their interactions and related pathways.Results A total of 43 target prescriptions were screened out.The property was mainly warm,followed by cold and flat.Themedicine taste was mainly sweet,followed by bitter and pungent.Spleen meridian,lung meridian,heart meridian andkidney meridian were the main meridians.The highest frequency of efficacy was complement-deficiency type,followedby astringent type and heat-clearing type.Four core drugs were combined with Panax Ginseng,frequency from high tolow being Ophiopogon,Baked Liquorice,Angelica sinensis and Donkey-hide Gelatin.There were 116 active componentsand 949 targets in the drug combination.ESR1,ESR2,CYP19A1 and PTPN1 were important targets.The activecomponents of core drugs mainly involved protein serine/threonine kinase activity,protein tyrosine kinase activity,nuclear receptor activity,ligand-activated transcription factor activity,etc.The potential targets were mainly located inmembrane raft,membrane microdomain,membrane regio

关 键 词：《温病条辨》 人参 数据挖掘 网络药理学 用药规律 

分 类 号：R285[医药卫生—中药学]