枸橼酸芬太尼对髋部骨折模型疼痛阈值和脊髓P物质信号传导的影响  

Effects of fentanyl citrate on pain threshold and spinal substance P signaling transduction in a hip fracture model

作　　者：冯晓利 孙智 刘俊英 汪丽珍 刘欣哲 FENG Xiaoli;SUN Zhi;LIU Junying;WANG Lizhen;LIU Xinzhe(Xingtai Central Hospital of Hebei Province,Xingtai,Hebei,054000)

机构地区：[1]河北省邢台市中心医院,河北邢台054000

出　　处：《实用临床医药杂志》2024年第21期89-94,99,共7页Journal of Clinical Medicine in Practice

基　　金：河北省邢台市重点研发计划自筹项目(2023ZC074)。

摘　　要：目的探讨枸橼酸芬太尼对髋部骨折老龄大鼠疼痛的影响及其作用机制。方法将30只髋部骨折的老龄大鼠分为模型组(n=10)、枸橼酸芬太尼组(n=10)和枸橼酸芬太尼+Sar-SP组(n=10),另取同期健康大鼠设为正常组(n=10)。枸橼酸芬太尼组大鼠尾静脉滴注10μg/kg枸橼酸芬太尼注射液,枸橼酸芬太尼+Sar-SP组大鼠鞘内注射10μL Sar-SP后进行枸橼酸芬太尼干预,正常组和模型组大鼠尾静脉滴注等剂量生理盐水。治疗前(0 h)以及治疗后1、6、24、168 h测量大鼠后爪疼痛阈值、承重值、后爪温度和背腹厚度。采用酶联免疫吸附测定(ELISA)检测大鼠脊髓组织中肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)、白细胞介素-1β(IL-1β)和谷氨酸(Glu)水平;采用免疫组化染色法观察大鼠脊髓组织中离子钙结合衔接分子1(Iba-1)、胶质纤维酸性蛋白(GFAP)和P物质(SP)阳性表达率;采用实时荧光定量聚合酶链反应(qRT-PCR)检测大鼠海马组织中SP和速激肽受体1(TACR1)mRNA相对表达水平;采用Western blot检测大鼠脊髓组织中波形蛋白(VIM)、核受体共阻遏蛋白1(NOCR)、睫状神经营养因子受体(CNTFR)和表皮生长因子受体(EGFR)蛋白相对表达量。结果与正常组比较,模型组大鼠治疗前0 h及治疗后1、6、24、168 h,后爪疼痛阈值和承重值降低,后爪温度升高,背腹厚度增加,且脊髓组织中TNF-α、IL-6、IL-1β和Glu水平,Iba-1和GFAP阳性表达率,SP阳性表达率,SP和TACR1mRNA相对表达水平以及VIM、NOCR、CNTFR和EGFR蛋白相对表达量升高,差异有统计学意义(P<0.05)。与模型组比较,枸橼酸芬太尼组大鼠治疗后1、6、24、168 h,后爪疼痛阈值和承重值升高,后爪温度降低,背腹厚度减小,且脊髓组织中TNF-α、IL-6、IL-1β和Glu水平,Iba-1和GFAP阳性表达率,SP阳性表达率,SP和TACR1mRNA相对表达水平以及VIM、NOCR、CNTFR和EGFR蛋白相对表达量降低,差异有统计学意义(P<0.05)。与枸橼酸芬Objective To investigate the effects of fentanyl citrate on pain in aged rats with hip fracture and its underlying mechanisms.Methods Thirty aged rats with hip fracture were divided into model group(n=10),fentanyl citrate group(n=10),and fentanyl citrate+Sar-SP group(n=10).Additionally,healthy rats from the same period were included as normal group(n=10).Rats in the fentanyl citrate group received intravenous tail injection of 10μg/kg fentanyl citrate,while those in the fentanyl citrate+Sar-SP group underwent intrathecal injection of 10μL Sar-SP for fentanyl citrate intervention.Rats in the normal and model groups received intravenous tail injection of an equal volume of saline.Pain threshold,weight-bearing capacity,temperature,and dorsoventral thickness of the hind paws were measured before treatment(0 h)and at 1,6,24,and 168 h post-treatment.Enzyme-linked immunosorbent assay(ELISA)was used to detect levels of tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),interleukin-1β(IL-1β),and glutamic acid(Glu)in the spinal cord tissue of rats.Immunohistochemical staining was employed to observe the positive expression rates of ionized calcium-binding adapter molecule 1(Iba-1),glial fibrillary acidic protein(GFAP),and substance P(SP)in the spinal cord tissue.Real-time fluorescent quantitative polymerase chain reaction(qRT-PCR)was conducted to detect the relative expression levels of SP and tachykinin receptor 1(TACR1)mRNA in the hippocampus tissue of rats.Western blot analysis was performed to assess the relative expression levels of vimentin(VIM),nuclear receptor corepressor 1(NOCR),ciliary neurotrophic factor receptor(CNTFR),and epidermal growth factor receptor(EGFR)proteins in the spinal cord tissue.Results Compared with the normal group,rats in the model group exhibited decreased pain threshold and weight-bearing capacity,increased hind paw temperature and dorsoventral thickness,as well as elevated levels of TNF-α,IL-6,IL-1β,and Glu,increased positive expression rates of Iba-1 and GFAP,heightened positive e

关 键 词：枸橼酸芬太尼 髋部骨折 老龄 疼痛 P物质 大鼠 

分 类 号：R683.3[医药卫生—骨科学] R453[医药卫生—外科学] R446[医药卫生—临床医学]