Stability and purity of selected ryegrass Epichloë endophytes in New Zealand dairy pastures  

作　　者：David E.Hume Dongwen Luo Grant M.Rennie Warren M.King Anna L.Taylor Marty J.Faville Katherine N.Tozer 

机构地区：[1]AgResearch Ltd.,Grasslands Research Centre,Palmerston North,New Zealand [2]AgResearch Ltd.,Ruakura Research Centre,Hamilton,New Zealand [3]AgResearch Ltd.,Lincoln Research Centre,Lincoln,New Zealand

出　　处：《Grassland Research》2024年第2期113-122,共10页草地研究（英文）

基　　金：DairyNZ;New Zealand Ministry for Agriculture and Forestry(MAF),Grant/Award Number:“Sustainable Farming Fund project 08/019–Best-Practice Pasture Renewal for Forage Production and Sustainability”;AgResearch,Grant/Award Number:Strategic Science Investment Fund。

摘　　要：Background:Perennial ryegrass(Lolium perenne)in New Zealand pastures is typically infected with the mutualist Epichloëfungal endophyte.This endophyte assists the plant in resisting biotic and abiotic stresses,but the standard strain of endophyte is toxic to livestock.Elite ryegrasses with selected endophytes have been developed to provide protective properties to the grass plant and lessen or eliminate the negative impacts on livestock.Methods:Using immunology and molecular techniques,the presence of endophyte infection and endophyte strain in ryegrass tillers was determined for 24 dairy pastures sampled for up to 7 years in regions of the North and South Islands.Results:In general,infection levels were high and showed small increases over time.Some pastures failed to reach 70%infection.The sown,selected endophytes were the dominant endophyte strains present and these were stable over time.Standard endophyte was the primary nonsown endophyte,and while generally low and so of little importance,it increased over time and for some pastures,this would have been detrimental to livestock.Pasture establishment technique influenced the level of contamination.Conclusions:Results reinforce the importance of following best practice procedures in the seed industry and on-farm.Researchers should monitor trials for contaminating nonsown standard endophyte.

关 键 词：competition Epichloë Lolium perenne pasture renewal selected endophytes toxicity 

分 类 号：S812[农业科学—草业科学]