Chromosome-scale genome assembly of the autoalloenneaploid Arundo donax  

作　　者：Mengmeng Ren Fupeng Liu Xiaohong Han Daohong Wu Hai Peng 

机构地区：[1]Wuhan Rundo Biotechnology Co.Ltd.,Wuhan,Hubei,China 2Institute for Systems Biology,Jianghan University,Wuhan,Hubei,China

出　　处：《Grassland Research》2024年第3期230-242,共13页草地研究（英文）

基　　金：Jianghan University,Grant/Award Number:2023XKZ024;National Forestry and Grassland Administration,Grant/Award Number:KJZXXP202314。

摘　　要：Background:Arundo donax L.has great potential as an energy crop due to its high biomass yield and broad adaptability,while lack of a reference genome is a hindrance to genetic improvement efforts for this species.Methods:Genome assembly of A.donax was conducted by utilizing PacBio SMRT sequencing and high-throughput chromosome conformation capture technology,with further analysis exploring the plant's ploidy,whole-genome duplication event,and evolutionary history through comparative genomics.Results:The genome assembly of A.donax consists of 1.30 Gb with a contig N50 size of 33.15 Mb.A total of 74403 gene models were identified,with over 90%of genes being functionally annotated.Karyotype and synteny analyses revealed that A.donax is an autoalloenneaploid(3n=9x=108)and has experienced significant gene family expansion and two whole-genome duplication events during its evolutionary history.Furthermore,utilizing the genome assembly,a variety of salinity stress-related genes were uncovered through the analysis of public RNA-seq data.Conclusions:This study presents the initial chromosome-scale genome assembly of A.donax,which will advance genetic comprehension and support the genetic enhancement of this important energy crop.

关 键 词：Arundo donax autoallopolyploid comparative genomics genome assembly 

分 类 号：Q78[生物学—分子生物学]