Effects of triclosan adsorption on intestinal toxicity and resistance gene expression in Xenopus tropicalis with different particle sizes of polystyrene  

作　　者：Zhuo Dai Zikai Wang Xinying Pan Li Zheng Yanbin Xu Qingxia Qiao 

机构地区：[1]Guangdong Key Laboratory of Environmental Catalysis and Health Risk Control,Guangzhou Key Laboratory Environmental Catalysis and Pollution Control,Institute of Environmental Health and Pollution Control,College of Environmental Science and Engineering,Guangdong University of Technology,Guangzhou 510006,China

出　　处：《Journal of Environmental Sciences》2024年第12期176-185,共10页环境科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(Nos.31802025,41977340,and 42277260).

摘　　要：Microplastics(MPs)are commonly found with hydrophobic contaminants in the water column and pose a serious threat to aquatic organisms.The effects of polystyrene microplastics of different particle sizes on the accumulation of triclosan in the gut of Xenopus tropicalis,its toxic effects,and the transmission of resistance genes were evaluated.The results showed that co-exposure to polystyrene(PS-MPs)adsorbed with triclosan(TCS)caused the accumulation of triclosan in the intestine with the following accumulation capacity:TCS+5μm PS group>TCS group>TCS+20μm PS group>TCS+0.1μm PS group.All experimental groups showed increased intestinal inflammation and antioxidant enzyme activity after 28 days of exposure to PS-MPs and TCS of different particle sizes.The TCS+20μm PS group exhibited the highest upregulated expression of pro-inflammatory factors(IL-10,IL-1β).The TCS+20μm group showed the highest increase in enzyme activity compared to the control group.PS-MPs and TCS,either alone or together,altered the composition of the intestinal microbial community.In addition,the presence of more antibiotic resistance genes than triclosan resistance genes significantly increased the expression of tetracycline resistance and sulfonamide resistance genes,which may be associated with the development of intestinal inflammation and oxidative stress.This study refines the aquatic ecotoxicity assessment of TCS adsorbed by MPs and provides informative information for the management and control of microplastics and non-antibiotic bacterial inhibitors.

关 键 词：POLYSTYRENE TRICLOSAN Xenopus-tropicalis Resistance genes Gut community 

分 类 号：X513[环境科学与工程—环境工程]