基于DNA分子标记及形态特征的温州市内高校常见园林竹种鉴定  

Identification of Common Bamboo Species in Universities in Wenzhou Based on DNA Molecular Marker and Morphology

作　　者：徐凯伦 伍桂芳 胡彩凤 金鑫杰 张永华[1] XU Kaiun;WU Guifang;HU Caifeng;JIN Xinjie;ZHANG Yonghua(College of Life and Environmental Sciences,Wenzhou University,Wenzhou,China 325035;School of Ecological and Environmental Sciences,East China Normal University,Shanghai,China 200241)

机构地区：[1]温州大学生命与环境科学学院,浙江温州325035 [2]华东师范大学生态与环境科学学院,上海200241

出　　处：《温州大学学报（自然科学版）》2024年第4期56-67,共12页Journal of Wenzhou University(Natural Science Edition)

基　　金：浙江省自然科学基金项目(LY21C030002);百山祖国家公园科学研究项目(2021KFLY06)。

摘　　要：竹类在表观形态上的高度相似性,导致了传统形态学方法难以实现准确的竹种鉴定.为探讨DNA分子标记在竹亚科植物分类鉴定中的适用性和准确性,本研究选取了两个稳定且多态的叶绿体DNA非编码区片段(trpl32-trnL和rpl16内含子),对温州市两所高校校园园林竹种进行了序列对比和系统进化分析,并结合形态特征,鉴定所研究的样本为3属11种.从系统发育分析可知,不同属的竹种非常稳健地单独分支聚类;在遗传距离上,竹种属间遗传距离远大于种间遗传距离.此外,仅从分子标记出发,该DNA序列片段组合在属阶元达到了100%的鉴别率,但在种阶元只有27.3%的鉴别率.综上,DNA分子标记可在属阶元对竹种进行准确鉴定,再结合竹种分类图鉴,能有效提高竹种鉴定准确性和效率.Due to the high similarity in morphology of bamboo species,it is very difficult to accurately identify bamboo species by traditional morphological methods.To explore the applicability and accuracy of DNA molecular marker in the taxonomic identification of Bambusoideae,two stable and polymorphic sequences of chloroplast DNA non-coding regions(trpl32-trnL and rpl16 intron)were selected for sequence identification and phylogenetic analysis of bamboo species in two universities,Wenzhou.Coupled with morphology,these samples could be identified as 11 species belonging to 3 genera of Bambusoideae.The phylogenetic analysis showed that bamboo species of different genera were very strongly separately clustered.In terms of genetic distance,the genetic distance among different genera was far greater than that among different species.Moreover,only based on molecular marker,the combination of DNA sequences achieved a 100%identification rate at the genus level but only 27.3%at the species level.Our study shows that DNA molecular marker can accurately identify bamboo species at the genus level,and combined with the illustrated encyclopedia of bamboos,it can effectively improve the accuracy and efficiency of bamboo species classification.

关 键 词：DNA条形码 竹亚科 遗传变异 分子鉴定 植物多样性 

分 类 号：Q949[生物学—植物学]