川牛膝片产地趁鲜加工及其可行性  

The fresh processing technology and its feasibility of Cyathulae Radix slices

作　　者：郭晓亮 穆森 段媛媛 胡昌强 黄浩 GUO Xiao-liang;MU Sen;DUAN Yuan-yuan;HU Chang-qiang;HUANG Hao(Institute of Chinese Herbal Medicines,Hubei Academy of Agricultural Sciences/Key Laboratory of Biology and Cultivation of Herb Medicine,Ministry of Agriculture and Rural Affairs,Enshi 445000,Hubei,China;Chizhengtang Pharmaceutical(Enshi)Co.,Ltd.,Enshi 445000,Hubei,China)

机构地区：[1]湖北省农业科学院中药材研究所/农业农村部中药材生物学与栽培重点实验室,湖北恩施445000 [2]持正堂药业恩施有限公司,湖北恩施445000

出　　处：《湖北农业科学》2024年第11期114-121,共8页Hubei Agricultural Sciences

基　　金：湖北省支持企业技术创新发展项目(2021BAB131);国家现代农业产业技术体系建设专项(CARS-21);恩施州研究与开发项目(D20210039)。

摘　　要：为了优化川牛膝(Cyathulae Radix)片产地趁鲜切制加工工艺并验证其可行性,采用正交试验设计,考察初干温度、初干程度、发汗时间及复干温度对川牛膝片质量与干燥时长的影响,并通过质量评价与代谢组分析验证工艺的可行性。结果表明,4个因素均对干燥时长、浸出物含量存在显著或极显著影响;初干温度对杯苋甾酮含量、前杯苋甾酮含量存在显著影响;复干温度对前杯苋甾酮含量存在显著影响。川牛膝片产地趁鲜切制加工最佳工艺:川牛膝鲜品切为单支后,先于50℃干燥至含水量41.2%,再发汗15~30 h、切片,后于70℃干燥至含水量不高于12.0%。趁鲜川牛膝片质量优于市售饮片(SSP)与传统川牛膝片(CTP)。趁鲜川牛膝片(CXP)与传统川牛膝片的化学成分相似性很高;相对于CTP,筛选出的36种差异代谢物中,CXP中有7种黄酮类化合物、5种皂苷类化合物含量上调;有20种黄酮类化合物、2种皂苷类化合物和2种甾酮类化合物含量下调。因此,川牛膝片产地趁鲜加工取代传统方法在一定程度上是可行的。In order to optimize the fresh processing technology of Cyathulae Radix slices,and verify its feasibility,the orthogonal experimental design was used to investigate the effects of initial drying temperature,initial drying degree,perspiration time and redrying temperature on the quality and drying time of Cyathulae Radix slices.The feasibility of the process was verified by quality evaluation and metabolome analysis.The results showed that the four factors all had significant or extremely significant effects on drying time and extract content;the initial drying temperature had significant effects on the content of cyasterone and precyasterone;the redrying temperature had significant effects on the content of precyasterone.The best process of fresh processing on origin of Cyathulae Radix slices was as follows:After the fresh product was cut into a single branch,it was initially dried at 50 ℃ to the water content of 41.2%,then sweated for 15~30 h,sliced,and then dried at 70 ℃ until the water content was not higher than 12.0%.The quality of the slices produced by fresh processing on origin was better than that of the slices available in the market(SSP)and the slices produced by traditional methods(CTP).The chemical composition of the slices produced by fresh processing and the slices produced by traditional methods was very similar.Compared with CTP,among the 36 different metabolites,the contents of 7 flavonoids and 5 saponins were up-regulated in CXP.The contents of 20 flavonoids,2 saponins and 2 steroids were down-regulated.Therefore,it was feasible to replace the traditional method with fresh processing on origin of Cyathulae Radix slices.

关 键 词：川牛膝(Cyathulae Radix)片 产地趁鲜加工 质量 代谢组 可行性 

分 类 号：R282[医药卫生—中药学]