针刺对SAMP8小鼠学习记忆能力和肠道菌群的影响  

Effect of Acupuncture on Learning and Memory Ability and Intestinal Flora of SAMP8 Mice

作　　者：邢文文[1] 李昂 张宁 刘奇 王梦静 XING Wenwen;LI Ang;ZHANG Ning;LIU Qi;WANG Mengjing(College of Basic Medical Sciences,Shaanxi University of Chinese Medicine,Xi’an 712046,China;College of Acupuncture and Moxibustion,Shaanxi University of Chinese Medicine,Xi’an 712046,China;Shaanxi Key Laboratory of Acupuncture and Herbal Medicine,Xi’an 712046,China)

机构地区：[1]陕西中医药大学基础医学院,陕西西安712046 [2]陕西中医药大学针灸推拿学院,陕西西安712046 [3]陕西省针药结合重点实验室,陕西西安712046

出　　处：《南京中医药大学学报》2024年第11期1200-1210,共11页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：国家自然科学基金面上项目(82074567);国家自然科学基金青年项目(82205284);咸阳市科技创新人才培养项目(L2023-CXNL-CXRC-009)。

摘　　要：目的探讨针刺对快速老化小鼠亚系(Senescence-accelerated mouse prone 8,SAMP8)小鼠的学习记忆能力和肠道菌群结构的影响。方法将18只6月龄SAMP8小鼠随机分为模型组和针刺组,每组9只,以9只同月龄SAMR1小鼠作为空白组。针刺组选取百会、涌泉穴针刺,每周5次,每次20 min,连续8周。采用Morris水迷宫检测每组小鼠学习记忆能力,免疫荧光法检测大脑皮层β-淀粉样蛋白(β-Amyloid protein,Aβ)表达,16S rDNA技术检测肠道菌群变化。结果水迷宫实验结果显示,与空白组比较,模型组小鼠学习记忆能力显著降低(P<0.05),针刺组小鼠学习记忆能力较模型组显著提高(P<0.05)。免疫荧光结果显示,与空白组比较,模型组大脑皮层Aβ阳性表达显著增加(P<0.01),可见明显Aβ斑块沉积;针刺组较模型组Aβ斑块明显减少(P<0.05)。Alpha多样性指数显示,与空白组比较,模型组Ace、Simpson值降低(P<0.05),Shannon值增高(P<0.05);与模型组相比,针刺组Ace、Chao值升高(P<0.05,P<0.01)。Beta多样性分析中主成分分析(PCA)、主坐标分析(PCoA)结果显示各组内样本相对聚集,各组间样本距离较远,Venn图显示空白组、模型组和针刺组中分别有664、689、649个OTUs。肠道菌群结构分析结果显示,与空白组比较,模型组拟杆菌门、瘤胃菌科、嗜木聚糖真杆菌丰度增加(P<0.05),梭杆菌科、未分类的梭杆菌科和卟啉菌属丰度下降(P<0.05);与模型组相比,针刺组拟杆菌门下降、嗜木聚糖真杆菌丰度下降(P<0.05),梭杆菌科、未分类的梭杆菌科和卟啉菌属丰度增加(P<0.05)。结论针刺可以减少SAMP8小鼠大脑皮层Aβ沉积,改善学习记忆能力,可能与调节肠道菌群结构、增加物种多样性有关。OBJECTIVE To explore the effect of acupuncture on the learning and memory ability and intestinal flora structure of senescence-accelerated mouse prone 8(SAMP8)mice.METHODS Eighteen 6-month-old SAMP8 mice were randomly divided into model group and acupuncture group,with 9 mice in each group,and 9 senescence-accelerated mouse resistant strain 1(SAMR1)mice of the same month as the normal group.The acupuncture group selected Baihui and Yongquan points for acupuncture,5 times a week,20 min each time,for 8 consecutive weeks.Morris water maze was used to detect the learning and memory ability of mice in each group,immunofluorescence method was used to detectβ-amyloid protein(Aβ)expression in the cerebral cortex,and 16S rDNA technology was used to detect changes in intestinal flora.RESULTS The results of the water maze experiment showed that compared with the normal group,the learning and memory abilities of the mice in the model group were significantly reduced(P<0.05),and the learning and memory abilities of the mice in the acupuncture group were significantly improved compared with the model group(P<0.05).Immunofluorescence results showed that compared with the normal group,the model group had increased positive expression of Aβin the cerebral cortex,and obvious Aβplaque deposition could be seen;the acupuncture group had fewer Aβplaques than the model group.Alpha diversity index showed that compared with the normal group,the Ace and Simpson values of the model group decreased(P<0.05)and the Shannon value increased(P<0.05);compared with the model group,the Ace and Chao values of the acupuncture group increased(P<0.05,P<0.01).In the beta diversity analysis,the results of principal component analysis(PCA)and principal coordinate analysis(PCoA)showed that the samples in each group were relatively clustered,and the distance between samples in each group was far away.The Venn diagram showed that there were 664,689,and 649 OTUs in the normal group,model group,and acupuncture group,respectively.Results of intestinal flor

关 键 词：阿尔茨海默病 针刺 肠道菌群 SAMP8 16S rDNA 

分 类 号：R285.5[医药卫生—中药学]