乙烯利处理菠萝不同成熟期果实中AcMYB103基因表达规律研究  

AcMYB103 Gene Expression Pattern Treated with Ethephon in Dif-ferent Ripening Stages of Ananas comosus

作　　者：韦巧云 张秀芬 韩骁 赵静 周彩霞 蒋娟娟 徐健 WEI Qiaoyun;ZHANG Xiufen;HAN Xiao;ZHAO Jing;ZHOU Caixia;JIANG Juanjuan;XU Jian(Guangxi South Subtropical Agricultural Research Institute,Longzhou,Guangxi 532415,China)

机构地区：[1]广西南亚热带农业科学研究所,广西龙州532415

出　　处：《热带作物学报》2024年第11期2277-2286,共10页Chinese Journal of Tropical Crops

基　　金：广西农业科学院基本科研业务费专项(桂农科2023YM128);广西农业科学院稳定资助科研团队项目(桂农科2021YT166)。

摘　　要：菠萝属于凤梨科(Bromeliaceae)凤梨属(Ananas Merr.)的一种重要热带水果,其花果发育理论与转录调控技术研究是菠萝产业的热点之一。乙烯是调控菠萝催花和果实发育的重要激素之一。菠萝基因组测序鉴定出94个菠萝R2R3-MYB转录因子,分为26亚族,其中S19亚族的MYB103与拟南芥等植物花药和叶片发育调控相关。为证明菠萝MYB103转录因子调控菠萝果实发育的潜在功能,从菠萝台农17号果实中克隆了1个MYB转录因子AcMYB103,全长1204 bp,编码区1038 bp,编码345个氨基酸。该基因编码蛋白含有2个典型的SANT结构域,定位在细胞核,无跨膜结构域和信号肽,是植物R2R3型MYB转录因子家族的一员。将菠萝和拟南芥MYB家族成员进行聚类分析发现,AcMYB103与拟南芥AtMYB103序列相似度最高,并与AtMYB80和AtMYB35聚类于S19亚族,据此命名为AcMYB103。进一步采用体积分数为0%、0.5%、1.0%和1.5%共4个乙烯利浓度处理台农17号、小目手撕和大目手撕3个品种小果期、膨大期和成熟期果实,并分析AcMYB103的表达量。结果表明,在小果期,随着乙烯利浓度的增加,台农17号AcMYB103表达规律呈下调模式,在小目手撕和大目手撕中呈上调模式。在膨大期,在台农17号和小目手撕品种中均呈现下调模式,在大目手撕果实中呈现上调模式。在成熟期果实中AcMYB103不表达,说明AcMYB103基因表达在乙烯利处理的品种和果实发育时期具有特异性,在果实发育早期起到差异化调控作用。本研究为深入研究AcMYB103基因在菠萝果实生长发育的调控机制奠定基础。Pineapple,an important tropical fruit,belongs to the family Bromeliaceae(Ananas Merr.),and its flower and fruit development theory and transcriptional regulation technology are one of the hot spots in the pineapple industry.Ethylene is one of the important hormones that regulate the flowering and fruit development of pineapple.In the study of watercore disorder in Bali pineapple varieties,it was found that two MYB transcription factors,AcMYB68 and Ac-MYB109,played an important role in fruit development.With the completion of pineapple genome sequencing,94 pi-neapple R2R3-MYB transcription factors have been identified in the pineapple genome,which are divided into 26 sub-families,and are differentially expressed by salt and other stresses and ABA hormones.In this study we proposed a scientific hypothesis that ethylene mediated MYB transcription factors to regulate the development of pineapple fruits.In order to elucidate the molecular regulatory mechanism,a MYB transcription factor AcMYB103 was cloned from the fruit of pineapple Tainong 17,with a total length of 1204 bp,a coding region of 1038 bp,and encoding 345 amino acids.The protein contains two typical SANT domains,localized to the nucleus,without transmembrane domain and signal peptide,and was a member of the plant R2R3 MYB transcription factor family.Cluster analysis of pineapple and Ara-bidopsis MYB family members showed that AcMYB103 had the highest similarity with Arabidopsis AtMYB80,At-MYB103 and AtMYB35,and belonged to the S19 subfamily.Furthermore,four ethephon concentrations(volume fraction)of 0%,0.5%,1.0%and 1.5%were used to treat the fruits at the small fruit stage,expansion stage and ripe stage of the three cultivars Tainong 17,Xiaomushousi and Damushousi,and the expression level of AcMYB103 was analyzed.The results showed that with the increase of ethephon concentration at the small fruit stage,the expression of AcMYB103 in Tainong 17 showed a down-regulated pattern,and upregulated in the Xiaomushousi and Damushousi mode.In the ex-pansion stage,t

关 键 词：菠萝 AcMYB103 乙烯利 表达 成熟期 

分 类 号：S668.3[农业科学—果树学]