内蒙古典型草原土壤真菌群落结构对季节土层交互的响应  

Responses of Soil Fungal Community Structure to Seasonal Soil Layer Interactions in Typical Grassland of Inner Mongolia

作　　者：聂佳明 张晓馥 张起迪 郭晓冬 蔡思萌 董永旺 刘慧艳 赵鸿彬 无 NIE Jia-ming;ZHANG Xiao-fu;ZHANG Qi-di;GUO Xiao-dong;CAI Si-meng;DONG Yong-wang;LIU Hui-yan;ZHAO Hong-bin;无(Inner Mongolia Agricultural University,Hohhot,Inner Mongolia 010010,China;Inner Mongolia Agricultural University/Key Laboratory of Wheat Germplasm Innovation and Utilization Autonomous Region Higher School,Hohhot,Inner Mongolia 010010,China;Inner Mongolia Agricultural University/Key Laboratory of Biological Manufacturing of Inner Mongolia Autonomous Region,Hohhot,Inner Mongolia 010010,China;Inner Mongolia Agricultural University/Key Laboratory of Grassland Resources of the Ministry of Education,Hohhot,Inner Mongolia 010010,China)

机构地区：[1]内蒙古农业大学,内蒙古呼和浩特010010 [2]内蒙古农业大学/麦类种质创新利用自治区高等学校重点实验室,内蒙古呼和浩特010010 [3]内蒙古农业大学/内蒙古自治区生物制造重点实验室,内蒙古呼和浩特010010 [4]内蒙古农业大学/草原资源教育部重点实验室,内蒙古呼和浩特010010

出　　处：《草地学报》2024年第11期3408-3416,共9页Acta Agrestia Sinica

基　　金：国家自然科学基金项目(31760154、32360025);内蒙古自然科学基金项目(2021MS03094);内蒙古自治区水利科技项目(NSK202405)资助。

摘　　要：为探究草原土壤真菌群落结构对季节与土层交互的响应规律及其影响因素,本研究以内蒙古典型草原土壤为研究对象,采用高通量测序测定不同生长季、不同土层的真菌群落结构特征,探讨真菌群落结构变化与土壤理化性质的关系。结果显示,土壤有机质、速效氮、速效磷和土壤体积含水量随土层的加深而显著降低(P<0.05),土壤温度随季节更替而显著降低(P<0.05)。季节与土层交互下土壤真菌丰度和多样性差异显著,在0~10 cm土层,真菌丰度OTU在秋季最高(1069.11),而真菌多样性Shannon指数在夏季最高(7.63)。土壤真菌群落结构显著改变,其中,在LDA>4水平上有28种真菌具有显著差异。同时,土壤真菌丰度和多样性与速效氮、速效磷、土壤体积含水量和土壤温度均呈显著正相关(P<0.05),与pH值和土壤有机质呈显著负相关(P<0.05)。本试验结果为草地资源的可持续利用以及草原生态系统的稳定性研究提供数据支持。To investigate the response patterns of soil fungal community structure to the interactions of seasonality and soil depth and identify influencing factors,a study was conducted on the soil of a typical grassland in Inner Mongolia.High-throughput sequencing was used to evaluate the characteristics of fungal community structure in different growing seasons and soil layers.The relationship between changes in fungal community structure and soil physicochemical properties was investigated.The results showed significant decreases in soil organic matter,available nitrogen,available phosphorus,and soil water content with the increase of soil depth(P<0.05),while soil temperature significantly decreased with changing seasons(P<0.05).Notably,the interactions of seasonality and soil depth led to significant differences in fungal abundance and diversity.In the 0-10 cm soil layer,fungal abundance(OTU)peaked in autumn(1069.11),while fungal diversity(Shannon index)reached the highest point in summer(7.63).The structure of the soil fungal community underwent significant changes,with 28 fungal taxa showing significant differences at an LDA>4 level.Furthermore,soil fungal abundance and diversity were significantly positively correlated with available nitrogen,available phosphorus,soil water content and soil temperature(P<0.05)while they were significantly negatively correlated with pH and soil organic matter(P<0.05).These experimental results provide data support for the sustainable utilization of grassland resources and the study of grassland ecosystem stability.

关 键 词：土壤真菌群落结构 季节与土层交互变化 土壤理化性质 典型草原 

分 类 号：S812.2[农业科学—草业科学]