不同耐低温乳酸菌添加剂对天然牧草青贮品质的影响  

Effects of Different Low-temperature Tolerant Lactic Acid Bacteria Additives on Natural Pasture Silage Fermentation

作　　者：珠拉 王志军 包健 刘明健 闫雨婷 贾玉山 娜日苏 格根图 ZHU La;WANG Zhi-jun;BAO Jian;LIU Ming-jian;YAN Yu-ting;JIA Yu-shan;NA Ri-su;GE Gen-tu(College of Grassland and Resources and Environment,Inner Mongolia Agricultural University Key Laboratory of Forage Cultivation and the Processing and Highly Efficient Utilization of the Ministry of Agriculture,Key Laboratory of Grassland Resources of the Ministry of Education,Hohhot,Inner Mongolia 010019,China;Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot,Inner Mongolia 010019,China)

机构地区：[1]内蒙古农业大学草原与资源环境学院/农业农村部饲草栽培、加工与高效利用重点实验室/草地资源教育部重点实验室,内蒙古呼和浩特010019 [2]内蒙古自治区农牧业科学院,内蒙古呼和浩特010019

出　　处：《草地学报》2024年第11期3610-3618,共9页Acta Agrestia Sinica

基　　金：功能性添加剂对全混合发酵日粮养分保存与养殖效果的调控(2022YFE0111000-3)资助。

摘　　要：为了探究前期筛选出耐低温乳酸菌对天然牧草青贮发酵的影响,本试验以天然牧草为原料,设置无添加剂、CH(商用菌株)、耐低温菌株Z906、L12、复合菌株ZL共5个处理,在室温、15℃、5℃下自然发酵60 d后进行营养成分、发酵品质、微生物数量的测定。最终结果表明:温度对天然牧草青贮过程有十分显著的影响,随着温度的降低,干物质、乳酸、乙酸含量、大肠杆菌数量有下降趋势,而可溶性碳水化合物、酸性洗涤纤维含量、乳酸菌数量有上升趋势。5℃下Z906处理组的干物质、粗蛋白质含量均最高,pH值最低,乳酸含量最高,未产生丁酸,氨态氮含量最低。V-Score评分等级为良好,排名第一,得分为93.07,青贮品质最优。综上所述,在北方较冷地区对天然草青贮,可添加Z906菌株的应用效果最好。In order to explore the impact of early screened low-temperature-tolerant lactic acid bacteria on natural pasture silage fermentation,this experiment used natural pasture as raw material and set up 5 treatments without additives,CH(commercial strain),low-temperature-resistant strain Z906,L12,and compound strain ZL.After 60 days of natural fermentation at room temperature,15℃,and 5℃the nutritional content,fermentation quality and microbial population were determined.The final results showed that temperature had a significant effect on the silage process of natural grass.As the temperature decreases,dry matter,lactic acid,acetic acid content,and the number of coliform bacteria had a downward trend,while water soluble carbohydrate,acid detergent fiber content,and the number of lactic acid bacteria had an upward trend.At 5℃,the Z906 treatment group had the highest dry matter and crude protein contents,the lowest pH value,the highest lactic acid content,no butyric acid production,and the lowest ammonia nitrogen content.The V-Score rating ranked first with a score of 93.07,and the silage quality was the best.In summary,the application of Z906 strain was the best for natural grass silage in cold regions of northern China.

关 键 词：天然牧草 耐低温乳酸菌 青贮 发酵品质 

分 类 号：S816.53[农业科学—饲料科学]