山东省滨州市不同年龄女性HPV高危型感染率及E6/E7 mRNA表达情况分析  

Analysis of HPV high-risk subtype infection rate and E6/E7 mRNA expression in women of different ages in Binzhou City,Shandong Province

作　　者：李娜[1] 杨田田 司秀文[1] 张海涛 LI Na;YANG Tiantian;SI Xiuwen;ZHANG Haitao(Department of Laboratory;Department of Neurosurgery,Affiliated Hospital of Binzhou Medical College,Binzhou,Shandong 256600,China)

机构地区：[1]滨州医学院附属医院检验科,山东滨州256600 [2]滨州医学院附属医院神经外科,山东滨州256600

出　　处：《现代医药卫生》2024年第22期3815-3820,共6页Journal of Modern Medicine & Health

基　　金：山东省自然科学基金青年项目(ZR2020QH104)。

摘　　要：目的探讨山东省滨州市不同年龄妇女人乳头瘤病毒(HPV)高危型感染率及HPV E6/E7 mRNA表达情况。方法选取2022年1-10月在该院妇产科就诊行HPV-DNA分型检测的女性患者10887例。按年龄分组(<30岁组2180例,30~<40岁组3222例,40~<50岁组3438例,≥50岁组2047例)并统计HPV-DNA分型检测结果。在入选患者中分层随机选取HPV-DNA 16型阳性女性患者250例,各年龄组50例,统计其HPV E6/E7 mRNA检测结果,比较不同年龄组女性患者HPV-DNA分型及HPV E6/E7 mRNA表达的差异。结果10887例女性患者中阳性1666例,感染率为15.3%,其中高危型阳性1393例,占83.6%(1393/1666),低危型感染273例,占16.4%(273/1666)。HPV高危型阳性分布排前5位者依次为HPV 16、52、58、33、18型。不同年龄组女性患者感染率不同,HPV感染年龄分布曲线呈“V”形。不同年龄HPV-DNA 16型阳性女性患者HPV E6/E7 mRNA阳性率不同,40~<50岁组、≥50岁组女性患者HPV E6/E7 mRNA阳性率均高于30~<40岁组,差异均有统计学意义(P<0.05)。HPV-58型E6/E7 mRNA阳性率、拷贝值均高于HPV 16、18、52型,差异均有统计学意义(P<0.05)。HPV高危型混合感染与单一感染E6/E7 mRNA表达存在差异。结论通过开展HPV-DNA筛查工作及研究了解本地区HPV亚型的分布具有非常重要的价值。年轻妇女和围绝经期及以上妇女HPV感染风险较高。随年龄增加HPV 16型感染人群中HPV E6/E7 mRN阳性率逐渐升高,即发生宫颈病变的风险增加。不同HPV高危型感染E6/E7 mRNA阳性率、拷贝值均存在差异,且HPV高危型混合感染者E6/E7 mRNA阳性率、拷贝值均高于单一感染者。Objective To investigate the distribution of HPV infection and the expression of HPV E6/E7 mRNA in women of different age groups in Binzhou City,Shandong Province.Methods A total of 10887 patients who have received HPV-DNA typing in the Department of Obstetrics and Gynecology of the Affiliated Hospital of Binzhou Medical University from January to October 2022 were selected.The results of HPV-DNA typing were grouped according to age group(<30 group 2180 cases,30-<40 group 3222 cases,40-<50 group 3438 cases,≥50 group 2047 cases).Patients with HPV-DNA 16 positive were randomly selected for the HPV E6/E7 mRNA test results,including 50 patients of all ages.The differences of HPV-DNA typing and HPV E6/E7 mRNA expression in different age groups were compared.Results Among 10887 female patients,1666 cases were positive,and the infection rate was 15.3%,among which 1393 cases were high-risk positive,accounting for 83.6%(1393/1666),and 273 cases were low-risk infection,accounting for 16.4%(273/1666).The top five HPV high-risk types were HPV types 16,52,58,33 and 18 in turn.The infection rate of female patients in different age groups is different,and the age distribution curve of HPV infection is"V".The positive rate of HPV E6/E7 mRNA in female patients with HPV-DNA type 16 was different at different ages.The positive rate of HPV E6/E7 mRNA in female patients aged 40-<50 years and≥50 years is higher than that in female patients aged 30-<40 years,the difference were statistically significant(P<0.05).The positive rate and copy value of HPV type 58 E6/E7 mRNA were higher than those of HPV type 16,18 and 52,the difference were statistically significant(P<0.05).There were differences in E6/E7 mRNA expression between high-risk HPV mixed infection and single infection.Conclusion It is of great value to understand the distribution of HPV subtypes in this area through HPV-DNA screening and research.Young women and perimenopausal and above women are at higher risk of HPV infection.With the increase of age,the positive rate of HP

关 键 词：人乳头瘤病毒 HPV-DNA 高危型感染 E6/E7 mRNA 宫颈病变 山东 

分 类 号：R373[医药卫生—病原生物学] R737.33[医药卫生—基础医学]