基于肾脏功能以及肠道屏障完整性探讨车前草对高尿酸血症大鼠的影响  

Exploring the effect of Plantago asiatica on hyperuricemia rats based on renal function and intestinal barrier integrity

作　　者：缪珊珊 赵婧 张迎召 马欣宇 张驲秋 冯杰 MIAO Shanshan;ZHAO Jing;ZHANG Yingzhao;MA Xinyu;ZHANG Riqiu;FENG Jie(Jiamusi University School of Clinical Medicine,Jiamusi 154000,China;Laboratory Department of the First Affiliated Hospital of Jiamusi University,Jiamusi 154003,China)

机构地区：[1]佳木斯大学临床医学院,黑龙江佳木斯154000 [2]佳木斯大学附属第一医院检验科,黑龙江佳木斯154003

出　　处：《黑龙江医药科学》2024年第6期1-5,共5页Heilongjiang Medicine and Pharmacy

基　　金：黑龙江省大学生创新创业训练计划项目,编号:202110222168;黑龙江省省属高等学校基本科研业务费科研项目,编号:2020-KYYWF-0293。

摘　　要：目的:研究高尿酸血症(HUA)大鼠模型在车前草水煎液干预下,机体的肾脏功能、肠道屏障完整性以及炎症因子的变化。方法:诱导HUA大鼠模型,实验结束后检测血清尿酸(UA)、肌酐(Cr)、尿素氮(BUN)、黄嘌呤氧化酶(XOD)、腺苷脱氨酶(ADA)的含量,检测肾脏尿酸转运蛋白ATP结合盒亚家族G成员2(ABCG2)、尿酸转运蛋白葡萄糖易化转运蛋白9(GLUT9)、炎症小体(NLRP3)、肿瘤坏死因子-α(TNF-α)和肠道组织紧密连接蛋白(Occludin)、紧密连接蛋白-1(Z_(O)-1)的含量。结果:车前草水煎液可降低HUA大鼠血清UA、CR、BUN水平(P<0.05),抑制XOD和ADA的活性(P<0.05),并增加ABCG2、Occludin和Zo-1的表达(P<0.05),降低NLRP3、TNF-α和GLUT9的表达(P<0.05)。结论:HUA大鼠模型出现炎症、肾和肠道功能损伤等症状,在车前草水煎液的干预下得到改善。Objective:To investigate the changes in metabolic levels and inflammatory factors in a rat model of hyperuricemia(HUA)under the intervention of Plantago asiatica decoction.Methods:The HUA rat model was induced,and after the experiment,the levels of serum uric acid(UA),creatinine(Cr),urea nitrogen(BUN),xanthine oxidase(XOD),adenosine deaminase(ADA)were detected.The levels of renal uric acid transporter ATP binding cassette subfamily G member 2(ABCG2),uric acid transporter glucose transporter 9(GLUT9),inflammasome(NLRP3),tumor necrosis factor-α(TNF-α),intestinal tissue tight junction protein(Occludin),and tight junction protein-1(Z_(O)-1)were also measured.Result:Plantago asiatica decoction can reduce the levels of serum UA,CR,and BUN in HUA rats(P<0.05),inhibit the activity of XOD and ADA(P<0.05),increase the expression of ABCG2,Occludin,and Zo-1(P<0.05),and reduce the expression of NLRP3,TNF-α,and GLUT9(P<0.05).Conclusion:The HUA rat model showed symptoms such as inflammation and renal and intestinal dysfunction,which were improved by intervention with Plantago asiatica decoction.

关 键 词：车前草水煎液 高尿酸血症 尿酸 

分 类 号：R285.5[医药卫生—中药学]