摩腹法对失眠大鼠肠道菌群及单胺类神经递质的影响  

Effects of abdominal massage on gut microbiota and monoamine neurotransmitters in rats with insomnia

作　　者：古来撒尔·艾克拜尔 卢旭昇 张雪枫 邱思淦 牙库甫·亚森 郭蕾 张晓婷[1] 刘俊昌[1,2,3] Gulaisaer·Aikebaier;LU Xusheng;ZHANG Xuefeng;QIU Sigan;Yakufu·Yasen;GUO Lei;ZHANG Xiaoting;LIU Junchang(Xinjiang Medical University,Urumqi 830017,China;Xinjiang Medical University Affiliated Hospital of Traditional Chinese Medicine,Urumqi 830000,China;Xinjiang Uygur Autonomous Region Academy of Traditional Chinese Medicine,Urumqi 830000,China)

机构地区：[1]新疆医科大学,乌鲁木齐830017 [2]新疆医科大学附属中医医院,乌鲁木齐830000 [3]新疆维吾尔自治区中医药研究院,乌鲁木齐830000

出　　处：《中华中医药杂志》2024年第11期6053-6060,共8页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：新疆维吾尔自治区研究生科研创新项目(No.XJ2022G187);新疆医科大学研究生创新创业项目(No.CXCY2022049);国家自然科学基金地区项目(No.81960906);新疆维吾尔自治区科技创新团队(天山创新团队)项目(No.2022TSYCTD0008)。

摘　　要：目的:探索摩腹法治疗失眠的作用机制,明确其是否是治疗失眠的核心手法。方法:72只雄性Wistar大鼠随机分为正常组、模型组、单纯摩腹组、传统摩腹疗法组、药物组及药物+单纯摩腹组,每组12只。模型组和各干预组大鼠腹腔注射PCPA制备失眠模型,造模成功后,正常组和模型组不作干预;单纯摩腹组在大鼠腹部行摩法治疗;传统摩腹疗法组行含摩腹在内的多种推拿手法并用的治疗手法;药物组灌胃酒石酸唑吡坦片(0.92 mg·kg^(-1)·d^(-1));药物+单纯摩腹组在药物组基础上给予摩腹治疗。每日1次,连续14 d。最后一次干预后,ELISA法检测各组大鼠下丘脑中5-羟色胺(5-HT)、多巴胺(DA)、去甲肾上腺素(NE)含量;采集大鼠新鲜粪便,采用16S rDNA高通量测序技术分析各组大鼠肠道菌群的组成结构、多样性、物种差异性,并与5-HT、DA、NE进行Spearman相关性分析。结果:与正常组比较,模型组大鼠下丘脑中5-HT、DA含量显著降低,NE含量显著升高(P<0.05)。与模型组比较,各干预组大鼠下丘脑中5-HT含量显著升高、NE含量显著降低(P<0.05),传统摩腹疗法组、药物组、药物+单纯摩腹组大鼠下丘脑内DA含量较模型组显著升高(P<0.05),单纯摩腹组、药物组大鼠Chao1指数显著升高(P<0.05),单纯摩腹组大鼠Shannon指数亦升高(P<0.05)。Beta多样性分析结果显示,正常组与模型组样本之间有明显的分离;在门水平上,大鼠的粪便微生物以拟杆菌门和厚壁菌门为主;属水平上以拟杆菌属、乳酸杆菌属等菌属为主;门和属水平上分别得到7个和102个差异显著性物种。5-HT与乳酸杆菌属呈显著正相关(r=0.268,P<0.05),与拟杆菌属呈显著负相关(r=-0.342,P<0.01);DA与拟杆菌属、阴性杆菌属呈显著负相关(r=-0.252,r=-0.268,P<0.05);NE与拟普雷沃氏菌属、阴性杆菌属呈显著正相关(r=0.309,P<0.01;r=0.273,P<0.05)。结论:摩腹法是治疗失眠的核心手法,其作用机制Objective:To explore the mechanism of effect of abdominal massage for insomnia and determine whether it is the core therapy for the treatment of insomnia.Methods:A total of 72 male Wistar rats were randomly divided into normal group(FK),model group(FM),simple abdominal group(FD),abdominal therapy group(FC),drug group(FY)and drug+simple abdominal group(FT),with 12 rats in each group.Insomnia models were prepared by intraperitoneally injecting PCPA in rats of FM group and each treatment group.After successful modelling,FK and FM groups were not intervened;FD group treated with abdominal massage in the whole abdomen position;FC group treated with various massage methods containing abdominal massage;FY group treated with Zolpidem tartrate tablets(0.92 mg·kg^(-1)·d^(-1))by instillation;FT group treated with abdominal massage based on drug group,given daily for 14 days.After the final intervention,5-hydroxytryptamine(5-HT),dopamine(DA)and norepinephrine(NE)levels were analyzed by ELISA in hypothalamus of the rats in each group.16S rDNA sequencing was used to analyse the structure,diversity and species differences of the gut microbiota in each group,and the Spearman correlation analysis was performed with 5-HT,DA and NE.Results:The 5-HT and DA levels were significantly reduced and the NE level was significantly increased in the hypothalamus of rats in FM group compared with FK group(P<0.05).Compared with the FM group,the 5-HT levels in the hypothalamus of rats in each treatment group were increased significantly and the NE levels were decreased(P<0.05),and the DA levels in the hypothalamus of rats in the FC,FY,and FT groups were increased significantly(P<0.05).The Chao1 index was significantly increased in both FD and FY groups compared to FM group(P<0.05),and Shannon index was increased in FD group(P<0.05).Beta diversity analyses showed that there was significant segregation between samples in FK and FM groups;At the phylum level,faecal microbiota of rats were dominated by Bacteroidetes and Firmicutes;The genus level

关 键 词：摩腹法 肠道菌群 单胺类神经递质 失眠 推拿 外治法 

分 类 号：R244.1[医药卫生—针灸推拿学]