高山杜鹃组培快繁关键技术研究  

Research on Key Techniques for Tissue Culture of Rhododendron lapponicum

作　　者：南雅琪 刘娟 齐宇 董飞[1] 王成鹏 马蕾[1] 王烨楠 吕晓惠 朱娇[1] NAN Ya-qi;LIU Juan;QI Yu(Institute of Leisure Agriculture,Shandong Academy of Agricultural Sciences/East China Urban Agriculture Key Laboratory of the Ministry of Agriculture and Rural Affairs/Shandong Engineering Research Center for Ecological Horticultural Plant Breeding,Jinan,Shandong 250100;College of Horticulture and Landscape Architecture,Tianjin Agricultural University,Tianjin 300000;Shandong Hongmei Horticultural Technology Co.,Ltd.,Rizhao,Shandong 276800)

机构地区：[1]山东省农业科学院休闲农业研究所/农业农村部华东都市农业重点实验室/生态园艺植物育种山东省工程研究中心,山东济南250100 [2]天津农学院园艺园林学院,天津300000 [3]山东红梅园艺科技有限公司,山东日照276800

出　　处：《安徽农业科学》2024年第22期42-46,共5页Journal of Anhui Agricultural Sciences

基　　金：山东省农业科学院国际合作平台培优项目(CXGC2023-G16);山东省农业科学院农业科技创新工程(CXGC2022A12);山东省重点研发计划重大科技创新工程(2021LZGC019)。

摘　　要：以高山杜鹃品种“23XXL”“5RE”及“6GR”的叶片、茎段、苞片为外植体,研究基本培养基、外植体生理年龄及植物生长调节剂对高山杜鹃外植体愈伤组织诱导的影响。结果表明,高山杜鹃外植体在WPM培养基和MS培养基上均能诱导出愈伤组织。愈伤诱导阶段,添加2.0~3.0 mg/L TDZ诱导愈伤效果好,其中外植体生理年龄为2W叶片诱导的愈伤组织生长较好,愈伤诱导率最高为100%;愈伤分化阶段,最适宜的愈伤组织分化激素配比为ZT 2.5 mg/L+NAA 0.5 mg/L和TDZ 1.5 mg/L+NAA 1.0 mg/L,其中外植体生理年龄为1M叶片在Z7培养基愈伤组织分化较好,平均芽分化数为0.92;生根阶段,培养基最佳激素配比IBA 0.5 mg/L+NAA 1.0~1.5 mg/L均适合高山杜鹃的3种品种。该研究结果为构建高效、稳定的高山杜鹃组培快繁体系提供科学依据,为进一步实现高山杜鹃产业化奠定基础。This study used the leaves,stem segments,and bracts of alpine rhododendron varieties‘23XXL’‘5RE’and‘6GR’as explants to investigate the effects of basic culture medium,physiological age of explants,and plant growth regulators on callus induction in alpine rhododendron explants.The results showed that the explants of alpine rhododendron could induce callus on both WPM and MS media.During the callus induction stage,adding 2.0-3.0 mg/L TDZ had a good effect on inducing callus.Among them,the callus induced by leaves with a physiological age of 2W had better growth,and the highest callus rate was 100%;at the stage of callus differentiation,the most suitable ratio of callus differentiation hormones was ZT 2.5 mg/L+NAA 0.5 mg/L and TDZ 1.5 mg/L+NAA 1.0 mg/L.Among them,the explant with a physiological age of 1M had better callus differentiation on Z7 medium,with an average number of bud differentiation of 0.92;during the rooting stage,the optimal hormone ratio of the culture medium was IBA 0.5 mg/L+NAA 1.0-1.5 mg/L,which were suitable for the three varieties of alpine rhododendron.The research results provide a scientific basis for constructing an efficient and stable tissue culture and rapid propagation system of alpine rhododendron,and lay a foundation for further realizing the industrialization of alpine rhododendron.

关 键 词：高山杜鹃 组织培养 外植体 植物生长调节剂 

分 类 号：S685.21[农业科学—观赏园艺]