盐度驯化对许氏平鲉钠钾ATP酶活性及鳃中AQP3基因表达量的影响  

Effects of salinity acclimation on Na^(+),K^(+)-ATPase activity and AQP3 gene expression in gill of Sebastes schlegelii

作　　者：化文原 由玉琢 张桠铭 孙荣桢 丁烨 张语诺 王伟 陈岩 HUA Wenyuan;YOU Yuzhuo;ZHANG Yaming;SUN Rongzhen;DING Ye;ZHANG Yunuo;WANG Wei;CHEN Yan(Key Laboratory of Applied Biology and Aquaculture of Northern Fishes in Liaoning Province,Dalian Ocean University,Dalian 116023,China)

机构地区：[1]大连海洋大学,辽宁省北方鱼类应用生物学与增养殖重点实验室,辽宁大连116023

出　　处：《安徽农学通报》2024年第22期36-42,共7页Anhui Agricultural Science Bulletin

基　　金：辽宁省教育厅高等学校基本科研项目(JYTZD2023038)。

摘　　要：为探究盐度驯化对许氏平鲉钠钾ATP酶(NKA)活性及鳃中AQP3基因表达量的影响,将140尾该鱼分为急性盐度驯化和慢性盐度驯化两部分试验。急性盐度驯化将其放入盐度为32‰、27‰、24‰、21‰、18‰、15‰、12‰和9‰的试验组中进行盐度胁迫,取鳃、肠及肾组织样测定NKA活性;慢性盐度驯化将其放入盐度为32‰、25‰、20‰、15‰、10‰和5‰的试验组中进行盐度胁迫,取鳃组织样测定AQP3的表达量。急性盐度驯化结果表明,除对照组外,鳃中NKA活性均明显高于肠和肾中NKA的活性(P<0.05),各个盐度组中肠和肾中NKA的活性差异无统计学意义(P>0.05)。慢性盐度驯化结果显示,在盐度胁迫达到4 h时,AQP3的表达量在25‰和20‰组出现峰值,随着盐度下降,表达量也随之下降;96 h时25‰组AQP3表达量逐渐恢复,而20‰组表达量仍为峰值,而后随盐度下降逐渐下降。综上,许氏平鲉可通过NKA和AQP3调节机体的渗透压平衡。To investigate the effects of salinity acclimation on NKA(Na^(+),K^(+)-ATPase)activity and gill AQP3 gene expression of 140 Sebastes schlegelii.The experiment was divided into two parts:acute salinity acclimation and chronic salinity acclimation.They were subjected to salinity stress in experimental groups with acute salinity of 32‰,27‰,24‰,21‰,18‰,15‰,12‰and 9‰.NKA activity in gill,intestine and kidney were measured.In chronic salinity acclimation,it was placed in experimental groups with salinities of 32‰,25‰,20‰,15‰,10‰and 5‰for salinity stress,and the expression of AQP3 in the gill was measured.The results of acute salinity acclimation showed that NKA activity in gill was significantly higher than that in intestine and kidney except control group(P<0.05),there was no statistical significance in NKA activity in midintestine and kidney in all salinity groups(P>0.05).The results of chronic salinity acclimation showed that AQP3 expression peaked in 25‰and 20‰groups when salinity stress reached 4 h,and decreased with the decrease of salinity.At 96 h,the expression of AQP3 in the 25‰group gradually recovered,while the expression of AQP3 in the 20‰group was still at its peak,and then gradually decreased with the decrease of salinity.In conclusion,the Sebastes schlegelii could regulate the osmolality of the body through NKA and AQP3.

关 键 词：许氏平鲉 盐度胁迫 Na^(+) K^(+)-ATPase AQP3基因 

分 类 号：S965.399[农业科学—水产养殖]