吕梁黑猪BACE2基因克隆及表达分析  

Cloning and expression analysis of BACE2 gene in Lyuliang black pig

作　　者：杨梓杰 张晋 赵丹 YANG Zijie;ZHANG Jin;ZHAO Dan(Jinzhong College of Information,Taigu 030800,China)

机构地区：[1]晋中信息学院,山西太谷030800

出　　处：《安徽农学通报》2024年第22期43-49,共7页Anhui Agricultural Science Bulletin

基　　金：山西省教育科学“十四五”规划课题“高质量教育体系下高校动医专业基础课程实验数字化教学探索与实践”(SZH-230078)。

摘　　要：为探讨吕梁黑猪BACE2基因特点和结构,本研究采用PCR技术对该基因进行克隆,并对所编码的蛋白质开展生物信息学分析,分析内容包括蛋白质的理化性质、信号肽、亲疏水性、跨膜结构、序列比对、二、三级结构预测、亚细胞定位预测,以及互作关系;通过实时荧光定量PCR(qPCR)技术,研究BACE2基因在吕梁黑猪的心脏、肝脏、脾脏、肺脏、肾脏、肌肉和脂肪组织中的表达情况。生物信息学结果显示,该黑猪BACE2基因的编码序列大小(CDS)为1545 bp,包含514个氨基酸;BACE2蛋白是一种疏水性蛋白,无信号肽,具有明显的跨膜作用,是一种非分泌型的蛋白质;序列比对发现吕梁黑猪同山羊、牛亲缘关系较近,同家鼠亲缘关系较远;蛋白的二级结构由无规则卷曲(49.03%)、α-螺旋(18.48%)、延伸连(27.24%)和β-转角(5.25%)构成,主要分布于内质网(55.6%)。qPCR结果显示,其肾脏组织中BACE2基因的表达水平最高,肌肉组织中的表达量最低。研究结果揭示了BACE2蛋白拥有的多种独特属性及BACE2基因的分布特点,为该蛋白更深层次的科学探究提供参考。In order to investigate the characteristics and structure of the BACE2 gene in Lyuliang black pigs,the BACE2 gene was cloned by PCR technology,and the encoded protein was analyzed by bioinformatics,including physical and chemical properties,signal peptide,hydrophobicity,transmembrane structure,sequence alignment,secondary and tertiary structure prediction,subcellular localization,and interaction.The expression of the BACE2 gene in the heart,liver,spleen,lung,kidney,muscle and adipose tissue of this pigs was measured by real-time quantitative PCR.The results of bioinformatics showed that the coding sequence(CDS)of BACE2 gene size was 1545 bp,containing 514 amino acids.BACE2 protein is a hydrophobic protein,no signal peptide,has obvious transmembrane function,and is a non-secretory protein.Sequence alignment showed that the pig had a close genetic relationship with goat and cattle,and the furthest genetic relationship with Mus musculus.The secondary structure of the protein was composed of random curl(49.03%),α-helix(18.48%),extension link(27.24%)andβ-corner(5.25%),mainly distributed in the endoplasmic reticulum(55.6%).qPCR results showed that the expression level of BACE2 gene was the higher in kidney tissue and the lower in muscle tissue of this pigs.The results revealed a variety of unique properties of BACE2 protein and the distribution characteristics of BACE2 gene,which provided a reference for further scientific exploration of this protein.

关 键 词：吕梁黑猪 BACE2基因 生物信息学 

分 类 号：S85[农业科学—兽医学]