葛根素对LPS诱导的人单核细胞炎症反应的下调作用  

Reducing Effects of Puerarin on Lipopolysaccharide-Inducedinflammatory Response in THP-1 Monocyte

作　　者：杨静纯 李良枝 郑智慧[1] 张华豪 江秀娟 YANG Jingchun;LI Liangzhi;ZHENG Zhihui;ZHANG Huahao;JIANG Xiujuan(Guangdong Provincial Hospital of Chinese Medicine,Guangzhou,Guangdong 510120,China;Haizhu District Institute for Food and Drug Control,Guangzhou,Guangdong 510120,China)

机构地区：[1]广东省中医院,广东广州510120 [2]广州市海珠区食品药品检验所,广东广州510120

出　　处：《今日药学》2024年第11期816-820,共5页Pharmacy Today

摘　　要：目的探究葛根素降低LPS诱导的人单核细胞THP-1炎症反应的作用。方法对经LPS诱导的炎症反应模型细胞添加葛根素进行处理,以Elisa检测模型细胞IL-1β、IL-6、TNF-α分泌量差异。结果经葛根素(浓度范围30~250μmol·L-1)处理后,与对照组相比,THP-1模型细胞IL-1β、IL-6、TNF-α的分泌量均显著下降。结论葛根素可显著下调人单核细胞经LPS诱导后的促炎因子分泌量,降低其炎症反应程度。OBJECTIVE To investigate the inhibitoryeffect of puerarin on the lipopolysaccharide(LPS)-induced inflammatory response in THP-1(human monocytes).METHODS THP-1 cells were induced by LPS to activate theinflammatory response and then treated with puerarin.After that secretion of interleukin 1β(IL-1β),IL-6,tumor necrosis factor alpha(TNF-α)were detected using the ELISA method.RESULTS The secretion of IL-1β,IL-6,and TNF-αin inflammatory THP-1 cells was significantly reduced in the puerarin-treated groups(30-250μmol·L-1)compared to the model group.CONCLUSION Puerarin can significantly reduce LPS-induced proinflammatory cytokine secretion in inflammatory THP-1 cells,therebylessening the severity of the inflammatory response.

关 键 词：葛根素 炎症反应 促炎因子 人单核细胞 

分 类 号：R285[医药卫生—中药学]