Charge-guided masking of a membrane-destabilizing peptide enables efficient endosomal escape for targeted intracellular delivery of proteins  

作　　者：Yan Zhao Haolin Jiang Hang Chen Jiazhen Yu Luyao Wang Wen Zhou Juanjuan Du 

机构地区：[1]School of Pharmaceutical Sciences,MOE Key Laboratory of Bioorganic Phosphorus Chemistry&Chemical Biology,Tsinghua University,Beijing 100084,China [2]Joint Graduate Program of Peking-Tsinghua-NIBS,School of Life Sciences,Tsinghua University,Beijing 100084,China [3]Academy for Advanced Interdisciplinary Studies(AAIS),Peking University-Tsinghua University-National Institute Biological Sciences(PTN)Joint Graduate Program,Peking University,Beijing 100871,China

出　　处：《Acta Pharmaceutica Sinica B》2024年第10期4478-4492,共15页药学学报（英文版）

基　　金：supported by supported by Beijing Municipal Science&Technology Commission(Z231100007223008,China);the National Key R&D Program of China(2017YFA0207900,China);Tsinghua University Initiative Scientific Research Program(2023Z11DSZ001,China);the Tsinghua-Peking Joint Center for Life Sciences.

摘　　要：Intracellular delivery of biologicals such as peptides,proteins,and nucleic acids presents a great opportunity for innovative therapeutics.However,the endosome entrapment remains a major bottleneck in the intracellular delivery of biomacromolecules,largely limiting their therapeutic potential.Here,we converted a cell-penetrating peptide(CPP),low molecular weight protamine(LMWP),to endosomal escape peptides(EEPs)by masking LMWP with a pH-responsive counter-ionic peptide.The resulting masked CPPs(mLMWP and mLMWP2)effectively promoted the escape of peptide/protein cargoes from endosomes into the cytoplasm.Consequential lysosome repair and lysophagy were initiated upon the endolysosomal leakage.Minimal reactive oxygen species(ROS)elevation or cell death was observed.Based on mLMWP2,we constructed an intracellular protein delivery system containing an antibody as a targeting module,mLMWP2 as an endosomal escape module,and the desired protein cargo.With the HER2-targeting delivery system,we efficiently translocated cyclization recombination enzyme(Cre)and BH3-interacting domain death agonist(BID)into the cytosol of HER2^(+)cells to exert their biological activity.Thereby,the modular delivery system shows its potential as a promising tool for scientific studies and therapeutic applications.

关 键 词：Endosomal escape Cytosolic delivery ANTIBODY pH responsiveness LMWP Cell-penetrating peptides Targeted delivery Electrostatic masking 

分 类 号：R943[医药卫生—药剂学]